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Literature DB >> 22528308

PCR detection of uncultured rumen bacteria.

Jaime A Rosero¹, Lenka Strosová, Jakub Mrázek, Kateřina Fliegerová, Jan Kopečný.

Abstract

16S rRNA sequences of ruminal uncultured bacterial clones from public databases were phylogenetically examined. The sequences were found to form two unique clusters not affiliated with any known bacterial species: cluster of unidentified sequences of free floating rumen fluid uncultured bacteria (FUB) and cluster of unidentified sequences of bacteria associated with rumen epithelium (AUB). A set of PCR primers targeting 16S rRNA of ruminal free uncultured bacteria and rumen epithelium adhering uncultured bacteria was designed based on these sequences. FUB primers were used for relative quantification of uncultured bacteria in ovine rumen samples. The effort to increase the population size of FUB group has been successful in sulfate reducing broth and culture media supplied with cellulose.

Entities: Chemical Species

Mesh：

Substances：
Culture Media
DNA Primers

Year: 2012 PMID： 22528308 DOI： 10.1007/s12223-012-0135-4

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

18 in total

1. Phylogenetic analysis of fiber-associated rumen bacterial community and PCR detection of uncultured bacteria.

Authors: Satoshi Koike; Sayo Yoshitani; Yasuo Kobayashi; Keiichi Tanaka
Journal: FEMS Microbiol Lett Date: 2003-12-05 Impact factor: 2.742

2. Bacterial diversity dynamics in rumen epithelium of wethers fed forage and mixed concentrate forage diets.

Authors: S Sadet-Bourgeteau; C Martin; D P Morgavi
Journal: Vet Microbiol Date: 2010-05-10 Impact factor: 3.293

3. Isolation of previously uncultured rumen bacteria by dilution to extinction using a new liquid culture medium.

Authors: Nikki Kenters; Gemma Henderson; Jeyamalar Jeyanathan; Sandra Kittelmann; Peter H Janssen
Journal: J Microbiol Methods Date: 2010-10-26 Impact factor: 2.363

Review 4. OLIGO 7 primer analysis software.

Authors: Wojciech Rychlik
Journal: Methods Mol Biol Date: 2007

1. Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta.

Authors: Durgadevi Aphale; Aarohi Kulkarni
Journal: Vet World Date: 2018-07-27

1 in total

PCR detection of uncultured rumen bacteria.

1. Phylogenetic analysis of fiber-associated rumen bacterial community and PCR detection of uncultured bacteria.

2. Bacterial diversity dynamics in rumen epithelium of wethers fed forage and mixed concentrate forage diets.

3. Isolation of previously uncultured rumen bacteria by dilution to extinction using a new liquid culture medium.

Review 4. OLIGO 7 primer analysis software.

Review 5. Metagenomics approaches in systems microbiology.

6. Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa.

7. Molecular monitoring and isolation of previously uncultured bacterial strains from the sheep rumen.

8. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria.

9. Diversity of insect intestinal microflora.

10. Proteiniclasticum ruminis gen. nov., sp. nov., a strictly anaerobic proteolytic bacterium isolated from yak rumen.

1. Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta.