| Literature DB >> 22528100 |
Reed B Wickner1, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, Herman K Edskes.
Abstract
We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.Entities:
Mesh：
Substances：
Year: 2012 PMID： 22528100 PMCID： PMC3479635 DOI： 10.1007/978-1-61779-551-0_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745