BACKGROUND: Gastro-enteritis is associated with significant mortality in low- and middle-income countries, and rotavirus and norovirus are the principal viral agents implicated. AIMS: To investigate the molecular epidemiology and burden of disease associated with rotavirus and norovirus in children attending a health centre in Ethiopia. METHODS: A cross-sectional study was conducted in children attending a health centre in Awassa, southern Ethiopia. Children with diarrhoea (cases) and without diarrhoea (controls) were recruited over a 6-month period from December 2008 to May 2009. Rotavirus was detected by ELISA and genotyped by RT-PCR, and norovirus was amplified by RT-PCR with genotyping by sequence analysis of RT-PCR products. RESULTS: Rotavirus was detected in 44/200 (22%) cases and in none of the controls, with genotypes G3P[6] (48%), G1P[8] (27%) and G2P[4] (7%) being the strains most commonly identified. Norovirus was detected in 16 (8%) of 200 cases and in 4 (7%) of 57 controls. Norovirus GII.3 was the strain most commonly detected (40%: 8/20) and ten other genotypes were also detected. CONCLUSION: Rotavirus is a common cause of severe gastro-enteritis in children in Ethiopia; however, norovirus occurred with a similar frequency in cases and controls. A globally uncommon strain type, G3P[6], predominated within the rotavirus strains detected.
BACKGROUND:Gastro-enteritis is associated with significant mortality in low- and middle-income countries, and rotavirus and norovirus are the principal viral agents implicated. AIMS: To investigate the molecular epidemiology and burden of disease associated with rotavirus and norovirus in children attending a health centre in Ethiopia. METHODS: A cross-sectional study was conducted in children attending a health centre in Awassa, southern Ethiopia. Children with diarrhoea (cases) and without diarrhoea (controls) were recruited over a 6-month period from December 2008 to May 2009. Rotavirus was detected by ELISA and genotyped by RT-PCR, and norovirus was amplified by RT-PCR with genotyping by sequence analysis of RT-PCR products. RESULTS:Rotavirus was detected in 44/200 (22%) cases and in none of the controls, with genotypes G3P[6] (48%), G1P[8] (27%) and G2P[4] (7%) being the strains most commonly identified. Norovirus was detected in 16 (8%) of 200 cases and in 4 (7%) of 57 controls. Norovirus GII.3 was the strain most commonly detected (40%: 8/20) and ten other genotypes were also detected. CONCLUSION:Rotavirus is a common cause of severe gastro-enteritis in children in Ethiopia; however, norovirus occurred with a similar frequency in cases and controls. A globally uncommon strain type, G3P[6], predominated within the rotavirus strains detected.
Authors: Lindsay R Grant; Katherine L O'Brien; Robert C Weatherholtz; Raymond Reid; Novalene Goklish; Mathuram Santosham; Umesh Parashar; Jan Vinjé Journal: PLoS One Date: 2017-01-03 Impact factor: 3.240
Authors: Lucy Thorne; Angela Nalwoga; Alexander J Mentzer; Alexis de Rougemont; Myra Hosmillo; Emily Webb; Margaret Nampiija; Allan Muhwezi; Tommy Carstensen; Deepti Gurdasani; Adrian V Hill; Manj S Sandhu; Alison Elliott; Ian Goodfellow Journal: J Infect Dis Date: 2018-07-24 Impact factor: 5.226
Authors: Elisabeth Heylen; Bibi Batoko Likele; Mark Zeller; Stijn Stevens; Sarah De Coster; Nádia Conceição-Neto; Christel Van Geet; Jan Jacobs; Dauly Ngbonda; Marc Van Ranst; Jelle Matthijnssens Journal: PLoS One Date: 2014-06-26 Impact factor: 3.240
Authors: Paul Koki Ndombo; Valantine N Ndze; Charles Fokunang; Taku Nadesh Ashukem; Angeline Boula; Mina N Kinkela; Corlins E Ndode; Mapaseka L Seheri; Michael D Bowen; Diane Waku-Kouomou; Mathew D Esona Journal: Virology (Lond) Date: 2017-07-25