Da-Long Guo1, Ju-Ping Zhang, Yi-Min Xue, Xiao-Gai Hou. 1. College of Forestry, Henan University of Science and Technology, Luoyang, People's Republic of China. guodalong@mail.haust.edu.cn
Abstract
PREMISE OF THE STUDY: Microsatellite markers were isolated and characterized from the genome of Momordica charantia (bitter melon) to be applied in studies of genetic diversity and population structure. METHODS AND RESULTS: Twenty-five microsatellite loci were isolated from the genome of bitter melon using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) method. Ten loci were polymorphic, and the number of alleles per locus ranged from three to seven, with the observed heterozygosity ranging from 0.46 to 0.65. The markers also amplified successfully in the related species M. cochinchinensis and Cucurbita pepo. CONCLUSIONS: These markers will have potential utility for applications in genetic diversity evaluation, molecular fingerprinting, identification, comparative genomics analysis, and genetic mapping in Momordica species, as well as in C. pepo.
PREMISE OF THE STUDY: Microsatellite markers were isolated and characterized from the genome of Momordica charantia (bitter melon) to be applied in studies of genetic diversity and population structure. METHODS AND RESULTS: Twenty-five microsatellite loci were isolated from the genome of bitter melon using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) method. Ten loci were polymorphic, and the number of alleles per locus ranged from three to seven, with the observed heterozygosity ranging from 0.46 to 0.65. The markers also amplified successfully in the related species M. cochinchinensis and Cucurbita pepo. CONCLUSIONS: These markers will have potential utility for applications in genetic diversity evaluation, molecular fingerprinting, identification, comparative genomics analysis, and genetic mapping in Momordica species, as well as in C. pepo.
Authors: Anjali Shukla; V K Singh; D R Bharadwaj; Rajesh Kumar; Ashutosh Rai; A K Rai; Raja Mugasimangalam; Sriram Parameswaran; Major Singh; P S Naik Journal: PLoS One Date: 2015-06-05 Impact factor: 3.240
Authors: P Gangadhara Rao; Tusar Kanti Behera; Ambika B Gaikwad; Anilabh Das Munshi; Gograj Singh Jat; G Boopalakrishnan Journal: Front Plant Sci Date: 2018-10-31 Impact factor: 5.753
Authors: P Gangadhara Rao; T K Behera; Ambika B Gaikwad; A D Munshi; Arpita Srivastava; G Boopalakrishnan Journal: Sci Rep Date: 2021-02-18 Impact factor: 4.379