Literature DB >> 22522581

The use of fish-derived cell lines for investigation of environmental contaminants.

Vivian R Dayeh1, Niels C Bols, Kristin Schirmer, Lucy E J Lee.   

Abstract

This unit describes protocols for growing salmonid cell lines and using them in in vitro toxicology studies. Cell viability of cultures is assessed with three indicator dyes: alamar blue for metabolic activity, CFDA-AM for membrane integrity, and neutral red for lysosomal activity. These protocols are essential tools for investigating environmental toxicity at the cellular level.

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Year:  2003        PMID： 22522581     DOI： 10.1002/0471140856.tx0105s15

Source DB:  PubMed          Journal:  Curr Protoc Toxicol        ISSN： 1934-9254


  5 in total

1.  Cytotoxicity, genotoxicity and oxidative stress of malachite green on the kidney and gill cell lines of freshwater air breathing fish Channa striata.

Authors:  S Abdul Majeed; K S N Nambi; G Taju; S Vimal; C Venkatesan; A S Sahul Hameed
Journal:  Environ Sci Pollut Res Int       Date:  2014-07-16       Impact factor: 4.223

2.  A cell line (HEW) from embryos of haddock (Melanogrammus aeglefinius) and its capacity to tolerate environmental extremes.

Authors:  Steve P Bryson; Elizabeth M Joyce; D John Martell; Lucy E J Lee; Shawn E Holt; Steve C Kales; Kazuhiro Fujiki; Brian Dixon; Niels C Bols
Journal:  Mar Biotechnol (NY)       Date:  2006-08-18       Impact factor: 3.619

3.  Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions.

Authors:  Jenny Maner; Michael Burkard; Juan Carlos Cassano; Susan M Bengtson Nash; Kristin Schirmer; Marc J-F Suter
Journal:  RSC Adv       Date:  2019-11-29       Impact factor: 4.036

4.  Discovery and Use of Long dsRNA Mediated RNA Interference to Stimulate Antiviral Protection in Interferon Competent Mammalian Cells.

Authors:  Shawna L Semple; Sarah K W Au; Rajesh A Jacob; Karen L Mossman; Stephanie J DeWitte-Orr
Journal:  Front Immunol       Date:  2022-05-06       Impact factor: 8.786

Review 5.  Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays.

Authors:  Sephra N Rampersad
Journal:  Sensors (Basel)       Date:  2012-09-10       Impact factor: 3.576
  5 in total

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