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Literature DB >> 22517076

Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.

Antonella Battisti¹, Michelle A Digman, Enrico Gratton, Barbara Storti, Fabio Beltram, Ranieri Bizzarri.

Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. This journal is © The Royal Society of Chemistry 2012

Entities: CellLine Chemical Disease Gene Mutation Species

Mesh：

Substances：
Luminescent Proteins

Year: 2012 PMID： 22517076 PMCID： PMC3742097 DOI： 10.1039/c2cc30373f

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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