Literature DB >> 2251248

Radiolabeling of Necator americanus third stage larvae with 75Se-methionine.

A A Siddiqui1, Y Lei, A C Fusco, B Salafsky.   

Abstract

Third stage larvae of Necator americanus were radiolabeled with 75Se-methionine by two methods. Larvae labeled in aqueous cultures contained 188 and 25 cpm per sheathed and ex-sheathed larva, respectively. Larvae labeled in coproculture incorporated 25 and 18 cpm per sheathed and ex-sheathed larva, respectively. All of the label was decayed in 5 days from larvae labeled in aqueous cultures, whereas appreciable amounts of radioactivity were still detectable at day 7 of chase period in coproculture labeled larvae.

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Year:  1990        PMID: 2251248     DOI: 10.1007/bf00931094

Source DB:  PubMed          Journal:  Parasitol Res        ISSN: 0932-0113            Impact factor:   2.289


  5 in total

1.  A simple method for the collection of Necator americanus larvae.

Authors:  J Mueller; B Ellenberger; A C Fusco; B Salafsky; A A Siddiqui
Journal:  J Helminthol       Date:  1989-03       Impact factor: 2.170

2.  Schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay and autoradiography.

Authors:  J R Georgi
Journal:  Parasitology       Date:  1982-04       Impact factor: 3.234

3.  Skin penetration by Necator americanus larvae.

Authors:  B E Matthews
Journal:  Z Parasitenkd       Date:  1982

4.  Radiolabeling of infective larvae of Haemonchus contortus (Nematoda: Trichostrongyloidea) with 75Se-methionine and their performance as tracers in sheep.

Authors:  J R Georgi; L F Le Jambre
Journal:  J Parasitol       Date:  1983-10       Impact factor: 1.276

5.  The effect of method of infection on the pathway of juvenile Strongyloides ratti in the host.

Authors:  P A Wilson; N E Simpson; D S Seaton
Journal:  J Helminthol       Date:  1986-06       Impact factor: 2.170

  5 in total

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