| Literature DB >> 2251239 |
R Böse1, R H Jacobson, K R Gale, D J Waltisbuhl, I G Wright.
Abstract
Two new enzyme-linked immunosorbent assays (ELISA) for the diagnosis of Babesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinant B. bovis antigen expressed in Escherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.Entities:
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Year: 1990 PMID: 2251239 PMCID: PMC7088413 DOI: 10.1007/bf00931081
Source DB: PubMed Journal: Parasitol Res ISSN: 0932-0113 Impact factor: 2.289