| Literature DB >> 22509803 |
Lin Shi1, Kurumi Fujioka, Jiying Sun, Aiko Kinomura, Toshiya Inaba, Tsuyoshi Ikura, Megu Ohtaki, Mitsuaki Yoshida, Yoshiaki Kodama, Gordon K Livingston, Kenji Kamiya, Satoshi Tashiro.
Abstract
The analysis of dicentric chromosomes in human peripheral blood lymphocytes (PBLs) by Giemsa staining is the most established method for biological dosimetry. However, this method requires a well-trained person because of the difficulty in detecting aberrations rapidly and accurately. Here, we applied a fluorescence in situ hybridization (FISH) technique, using telomere and centromere peptide nucleic acid (PNA) probes, to solve the problem of biological dosimetry in radiation emergency medicine. A comparison by a well-trained observer found that FISH analysis of PBLs for the dose estimation was more accurate than the conventional Giemsa analysis, especially in samples irradiated at high doses. These results show that FISH analysis with centromeric/telomeric PNA probes could become the standard method for biological dosimetry in radiation emergency medicine.Entities:
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Year: 2012 PMID： 22509803 DOI： 10.1667/rr2849.1
Source DB: PubMed Journal: Radiat Res ISSN： 0033-7587 Impact factor: 2.841