OBJECTIVE: To use intraoperative confocal endomicroscopy for early detection and resection of squamous cell carcinoma (SCC) of the head and neck. A preliminary report. DESIGN: Prospective case series. SETTING: Tertiary referral hospital. PATIENTS: Fifteen consecutive patients with SCC of the oral cavity, hypopharynx, and larynx were included from the Department of Otolaryngology-Head and Neck Surgery, HSK Dr Horst Schmidt Kliniken GmbH, Wiesbaden, Germany INTERVENTIONS: Confocal endomicroscopy was performed during diagnostic and therapeutic procedures with a prototype of a rigid laser endoscope in combination with the already available technology of autofluorescence. MAIN OUTCOME MEASURES: Real-time visualization of cellular and subcellular details during endoscopy. Diagnostic scores were applied to differentiate dysplastic and malignant mucosal changes of SCC of the head and neck from normal squamous cell mucosa using this method. Results were correlated with the well-established gold standard, histologic analysis. RESULTS: Dysplastic and malignant changes of head and neck squamous cell mucosa were endoscopically determined by this unique in vivo application of confocal laser endomicroscopy using a rigid probe. CONCLUSIONS: We present preliminary and descriptive data using this novel technology in vivo. Considering the impact of a virtual real-time histologic analysis, this technology points to a very promising development. It may carry potential for quicker intraoperative diagnosis, less need for multiple frozen sections, and more precise resection margins.
OBJECTIVE: To use intraoperative confocal endomicroscopy for early detection and resection of squamous cell carcinoma (SCC) of the head and neck. A preliminary report. DESIGN: Prospective case series. SETTING: Tertiary referral hospital. PATIENTS: Fifteen consecutive patients with SCC of the oral cavity, hypopharynx, and larynx were included from the Department of Otolaryngology-Head and Neck Surgery, HSK Dr Horst Schmidt Kliniken GmbH, Wiesbaden, Germany INTERVENTIONS: Confocal endomicroscopy was performed during diagnostic and therapeutic procedures with a prototype of a rigid laser endoscope in combination with the already available technology of autofluorescence. MAIN OUTCOME MEASURES: Real-time visualization of cellular and subcellular details during endoscopy. Diagnostic scores were applied to differentiate dysplastic and malignant mucosal changes of SCC of the head and neck from normal squamous cell mucosa using this method. Results were correlated with the well-established gold standard, histologic analysis. RESULTS:Dysplastic and malignant changes of head and neck squamous cell mucosa were endoscopically determined by this unique in vivo application of confocal laser endomicroscopy using a rigid probe. CONCLUSIONS: We present preliminary and descriptive data using this novel technology in vivo. Considering the impact of a virtual real-time histologic analysis, this technology points to a very promising development. It may carry potential for quicker intraoperative diagnosis, less need for multiple frozen sections, and more precise resection margins.
Authors: Sneha Sethi; Xiangqun Ju; Richard M Logan; Paul Sambrook; Robert A McLaughlin; Lisa M Jamieson Journal: Int J Environ Res Public Health Date: 2021-11-25 Impact factor: 3.390
Authors: Nicolai Oetter; Christian Knipfer; Maximilian Rohde; Cornelius von Wilmowsky; Andreas Maier; Kathrin Brunner; Werner Adler; Friedrich-Wilhelm Neukam; Helmut Neumann; Florian Stelzle Journal: J Transl Med Date: 2016-06-03 Impact factor: 5.531