| Literature DB >> 22503880 |
Haixia Tian1, Guoqiang Zhuang, Anzhou Ma, Chuanyong Jing.
Abstract
The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera (Arthrobacter, Pseudomonas, Sphingomonas, and Acinetobacter). The bacteria were separated into cell wall and protoplast in the study to assess the As(V) attack. The As(V) stress on bacteria could be identified with SERS, but not with FTIR. The bacteria in our study primarily resist As(V) through sequestration of As(V) by the cell wall. The change in SERS peaks and their relationships with cell wall suggested that As(V) mainly interacts with functional groups on the cell wall including polysaccharides and flavin derivates.Entities:
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Year: 2012 PMID： 22503880 DOI： 10.1016/j.mimet.2012.03.018
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363