Literature DB >> 2249979

Dicyclohexylcarbodiimide as inducer of mitochondrial Ca2+ release.

E Chávez1, C Zazueta, E Díaz.   

Abstract

The effect of the alkylating reagent dicyclohexylcarbodiimide (DCCD) on mitochondrial Ca2+ content was studied. The results obtained indicate that DCCD at a concentration of 100 microM induces mitochondrial Ca2+ efflux. This reaction is accompanied by an increasing energy drain on the system, stimulation of oxygen consumption, and mitochondrial swelling. These DCCD effects can be partially suppressed by supplementing the incubation medium with 1 mM phosphate. By electrophoretic analysis on polyacrylamide-sodium dodecyl sulfate, it was found that DCCD binds to a membrane component with an Mr of 20 to 29 kDa.

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Year:  1990        PMID： 2249979     DOI： 10.1007/bf00809071

Source DB:  PubMed          Journal:  J Bioenerg Biomembr        ISSN： 0145-479X            Impact factor:   2.945


  33 in total

1.  STOICHIOMETRY OF RESPIRATORY STIMULATION, ACCUMULATION OF CA++ AND PHOSPHATE, AND OXIDATIVE PHOSPHORYLATION IN RAT LIVER MITOCHONDRIA.

Authors:  C S ROSSI; A L LEHNINGER
Journal:  J Biol Chem       Date:  1964-11       Impact factor: 5.157

2.  Characterization by Hg2+ of two different pathways for mitochondrial Ca2+ release.

Authors:  E Chávez; C Zazueta; E Díaz; J A Holquín
Journal:  Biochim Biophys Acta       Date:  1989-11-17

3.  Safranine as a probe of the mitochondrial membrane potential.

Authors:  K E Akerman; M K Wikström
Journal:  FEBS Lett       Date:  1976-10-01       Impact factor: 4.124

4.  Mitochondrial calcium release as induced by Hg2+.

Authors:  E Chávez; J A Holguín
Journal:  J Biol Chem       Date:  1988-03-15       Impact factor: 5.157

5.  Regulation of Ca2+ release from mitochondria by the oxidation-reduction state of pyridine nucleotides.

Authors:  A L Lehninger; A Vercesi; E A Bababunmi
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

6.  Temperature dependence of the atractyloside-induced mitochondrial Ca2+ release.

Authors:  E Chávez; A Osornio
Journal:  Int J Biochem       Date:  1988

7.  Control of mitochondrial Ca2+ retention by ADP-stimulated glutamic dehydrogenase.

Authors:  E Chávez; D Jay
Journal:  J Bioenerg Biomembr       Date:  1987-10       Impact factor: 2.945

8.  The effect of phosphoenolpyruvate on calcium transport by mitochondria.

Authors:  P Chudapongse; N Haugaard
Journal:  Biochim Biophys Acta       Date:  1973-05-25

9.  Energy transduction by the reconstituted b-c1 complex from yeast mitochondria. Inhibitory effects of dicyclohexylcarbodiimide.

Authors:  D S Beattie; A Villalobo
Journal:  J Biol Chem       Date:  1982-12-25       Impact factor: 5.157

10.  Effects of Pb2+ added in vitro on Ca2+ movements in isolated mitochondria and slices of rat kidney cortex.

Authors:  S C Kapoor; G D van Rossum
Journal:  Biochem Pharmacol       Date:  1984-06-01       Impact factor: 5.858
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  1 in total

1.  Two critical factors affecting the release of mitochondrial cytochrome C as revealed by studies using N,N'-dicyclohexylcarbodiimide as an atypical inducer of permeability transition.

Authors:  Takenori Yamamoto; Satsuki Terauchi; Aiko Tachikawa; Kikuji Yamashita; Masatoshi Kataoka; Hiroshi Terada; Yasuo Shinohara
Journal:  J Bioenerg Biomembr       Date:  2005-10       Impact factor: 2.945
  1 in total

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