R Bieżanowska-Kopeć1, T Leszczyńska. 1. Department of Human Nutrition, Faculty of Food Technology, Agricultural University of Krakow, Krakow.
Abstract
OBJECTIVES: The studies were carried out on 36 growing albino Wistar rats. PARTICIPANTS/MEASUREMENTS: The animals were randomly divided into six equinumerous groups (six rats per group), and were fed six different diets for 42 days. The control group (I) was fed with AIN-93G semi-synthetic diet, whereas groups II-VI were fed with AIN-93G semi-synthetic diet supplemented with: 2, 4, 8, 16 and 32 g of methionine/kg diet, respectively. There were assessed enzymatically, in rats' blood serum, the contents of homocysteine, total cholesterol, HDL fraction and triacyloglicerols. In addition, the LDL+VLDL cholesterol content was calculated. RESULTS: The methionine content of the diet was found to be highly positively correlated with the homocysteine content (r = 0.981) and negatively correlated with the triacylglycerols content (r = -0.916) of the experimental animals' blood serum. CONCLUSION: In the blood serum of rats fed the highest-methionine diet (32 g methionine/kg diet), the homocysteine content was significantly higher, as were the levels of total cholesterol and its HDL fraction, while the triacylglycerols content was lower as compared to the values obtained for rats fed other diet types.
OBJECTIVES: The studies were carried out on 36 growing albino Wistar rats. PARTICIPANTS/MEASUREMENTS: The animals were randomly divided into six equinumerous groups (six rats per group), and were fed six different diets for 42 days. The control group (I) was fed with AIN-93G semi-synthetic diet, whereas groups II-VI were fed with AIN-93G semi-synthetic diet supplemented with: 2, 4, 8, 16 and 32 g of methionine/kg diet, respectively. There were assessed enzymatically, in rats' blood serum, the contents of homocysteine, total cholesterol, HDL fraction and triacyloglicerols. In addition, the LDL+VLDL cholesterol content was calculated. RESULTS: The methionine content of the diet was found to be highly positively correlated with the homocysteine content (r = 0.981) and negatively correlated with the triacylglycerols content (r = -0.916) of the experimental animals' blood serum. CONCLUSION: In the blood serum of rats fed the highest-methionine diet (32 g methionine/kg diet), the homocysteine content was significantly higher, as were the levels of total cholesterol and its HDL fraction, while the triacylglycerols content was lower as compared to the values obtained for rats fed other diet types.
Authors: J Duan; T Murohara; H Ikeda; K Sasaki; S Shintani; T Akita; T Shimada; T Imaizumi Journal: Arterioscler Thromb Vasc Biol Date: 2000-12 Impact factor: 8.311
Authors: Anna Sierakowska-Fijałek; Piotr Kaczmarek; Lech Pokoca; Ireneusz Smorag; Marzenna Wosik-Erenbek; Zbigniew Baj Journal: Pol Merkur Lekarski Date: 2007-02
Authors: R B Kostogrys; C Johann; I Czyżyńska; M Franczyk-Żarów; A Drahun; E Maślak; A Jasztal; M Gajda; Ł Mateuszuk; T P Wrobel; M Baranska; I Wybrańska; K Jezkova; P Nachtigal; S Chlopicki Journal: J Nutr Health Aging Date: 2015-08 Impact factor: 4.075