| Literature DB >> 22497892 |
Takuya Yamashita1, Kazuya Nagano, So-ichiro Kanasaki, Yuka Maeda, Takeshi Furuya, Masaki Inoue, Hiromi Nabeshi, Tomoaki Yoshikawa, Yasuo Yoshioka, Norio Itoh, Yasuhiro Abe, Haruhiko Kamada, Yasuo Tsutsumi, Shin-ichi Tsunoda.
Abstract
Mesothelioma is a highly malignant tumor with a poor prognosis and limited treatment options. Although cisplatin (CDDP) is an effective anticancer drug, its response rate is only 20%. Therefore, discovery of biomarkers is desirable to distinguish the CDDP-susceptible versus resistant cases. To this end, differential proteome analysis was performed to distinguish between mesothelioma cells of different CDDP susceptibilities, and this revealed that expression of annexin A4 (ANXA4) protein was higher in CDDP-resistant cells than in CDDP-susceptible cells. Furthermore, ANXA4 expression levels were higher in human clinical malignant mesothelioma tissues than in benign mesothelioma and normal mesothelial tissues. Finally, increased susceptibility was observed following gene knockdown of ANXA4 in mesothelioma cells, whereas the opposite effect was observed following transfection of an ANXA4 plasmid. These results suggest that ANXA4 has a regulatory function related to the cisplatin susceptibility of mesothelioma cells and that it could be a biomarker for CDDP susceptibility in pathological diagnoses.Entities:
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Year: 2012 PMID： 22497892 DOI： 10.1016/j.bbrc.2012.03.144
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575