Literature DB >> 22497887

Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.

Bing Liu1, James A Garnett, Wei-chao Lee, Jing Lin, Paula Salgado, Jonathan Taylor, Yingqi Xu, Sebastian Lambert, Ernesto Cota, Steve Matthews.   

Abstract

The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 22497887      PMCID： PMC3522111          DOI： 10.1016/j.bbrc.2012.03.136

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  34 in total

1.  H/D isotope effects in protein thermal denaturation: the case of bovine serum albumin.

Authors:  Ling Fu; Sandrine Villette; Stéphane Petoud; Felix Fernandez-Alonso; Marie-Louise Saboungi
Journal:  J Phys Chem B       Date:  2011-02-03       Impact factor: 2.991

2.  Suppression of microtubule dynamic instability and treadmilling by deuterium oxide.

Authors:  D Panda; G Chakrabarti; J Hudson; K Pigg; H P Miller; L Wilson; R H Himes
Journal:  Biochemistry       Date:  2000-05-02       Impact factor: 3.162

3.  Solvent isotope effect and protein stability.

Authors:  G I Makhatadze; G M Clore; A M Gronenborn
Journal:  Nat Struct Biol       Date:  1995-10

4.  Characterization of three fimbrial genes, sefABC, of Salmonella enteritidis.

Authors:  S C Clouthier; K H Müller; J L Doran; S K Collinson; W W Kay
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490

Review 5.  Structural biology of the chaperone-usher pathway of pilus biogenesis.

Authors:  Gabriel Waksman; Scott J Hultgren
Journal:  Nat Rev Microbiol       Date:  2009-10-12       Impact factor: 60.633

6.  Hydrogen bond dynamics in the active site of photoactive yellow protein.

Authors:  Paul A Sigala; Mark A Tsuchida; Daniel Herschlag
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-26       Impact factor: 11.205

7.  Molecular dynamics of hydrogen bonds in protein-D2O: the solvent isotope effect.

Authors:  Sheh-Yi Sheu; E W Schlag; H L Selzle; Dah-Yen Yang
Journal:  J Phys Chem A       Date:  2008-01-15       Impact factor: 2.781

8.  The solution structure of the invasive tip complex from Afa/Dr fibrils.

Authors:  Ernesto Cota; Celine Jones; Peter Simpson; Harri Altroff; Kirstine L Anderson; Laurence du Merle; Julie Guignot; Alain Servin; Chantal Le Bouguénec; Helen Mardon; Stephen Matthews
Journal:  Mol Microbiol       Date:  2006-09-08       Impact factor: 3.501

9.  Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism.

Authors:  Han Remaut; Rebecca J Rose; Thomas J Hannan; Scott J Hultgren; Sheena E Radford; Alison E Ashcroft; Gabriel Waksman
Journal:  Mol Cell       Date:  2006-06-23       Impact factor: 17.970

Review 10.  Scaling and assessment of data quality.

Authors:  Philip Evans
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14
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  2 in total

1.  Effects of Excipient Interactions on the State of the Freeze-Concentrate and Protein Stability.

Authors:  Sampreeti Jena; Jacqueline Horn; Raj Suryanarayanan; Wolfgang Friess; Alptekin Aksan
Journal:  Pharm Res       Date:  2016-12-15       Impact factor: 4.200

2.  Crystal structure and analysis of HdaB: The enteroaggregative Escherichia coli AAF/IV pilus tip protein.

Authors:  Wei-Chao Lee; Steve Matthews; James A Garnett
Journal:  Protein Sci       Date:  2016-08-01       Impact factor: 6.725
  2 in total

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