| Literature DB >> 22494070 |
Weina Jin1, Qinghua Li, Jian Wang, Guoqiang Chang, Yani Lin, Huawen Li, Lihong Wang, Wei Gao, Tianxiang Pang.
Abstract
The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and marked IL-8 (interleukin-8) release. Inhibition of NHE1 under hypoxia additionally enhanced the level of C/EBPα and further promoted leukaemic cells differentiation. Pharmacological inhibition of p38 MAPK (mitogen-activated protein kinase) also significantly suppressed C/EBPα expression under hypoxia conditions after NHE1 inhibition. These results indicate the enhancement of hypoxia-induced K562 differentiation by NHE1 inhibition, which may be due to up-regulation of C/EBPα via p38 MAPK signalling pathway, which suggests a possible therapeutic target of NHE1 under hypoxia microenvironment in the treatment of leukaemic diseases.Entities:
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Year: 2012 PMID： 22494070 DOI： 10.1042/CBI20100919
Source DB: PubMed Journal: Cell Biol Int ISSN： 1065-6995 Impact factor: 3.612