Literature DB >> 224935

Improved in vitro light activation and assay systems for two spinach chloroplast enzymes.

P Schürmann, J P Jacquot.   

Abstract

An improved system for the in vitro light activation of the chloroplast enzymes fructose-1,6-bisphosphatase and NADP-dependent malate dehydrogenase is described. Through the presence of the monothiol beta-mercaptoethanol in the reaction mixtures the activated forms of the enzymes can be stabilized and their activity determined spectrophotometrically.

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Year:  1979        PMID： 224935     DOI： 10.1016/0005-2744(79)90067-6

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  Reduction of ferredoxin:thioredoxin reductase by artificial electron donors.

Authors:  P Schürmann; A L Stritt-Etter; J Li
Journal:  Photosynth Res       Date:  1995-11       Impact factor: 3.573

2.  Energetic aspects of the light activation of two chloroplast enzymes: fructose-1,6-bisphosphatase and NADP-malate dehydrogenase.

Authors:  M Miginiac-Maslow; J P Jacquot; M Droux
Journal:  Photosynth Res       Date:  1985-09       Impact factor: 3.573

3.  Modulation of NADP-Malate Dehydrogenase Activity in Maize Mesophyll Chloroplasts.

Authors:  R C Leegood; D A Walker
Journal:  Plant Physiol       Date:  1983-03       Impact factor: 8.340

4.  Regulation of photosynthetic carbon reduction cycle by ribulose bisphosphate and phosphoglyceric Acid.

Authors:  J C Servaites; W J Shieh; D R Geiger
Journal:  Plant Physiol       Date:  1991-11       Impact factor: 8.340

5.  Enzyme Regulation in C(4) Photosynthesis : PURIFICATION AND PROPERTIES OF THIOREDOXIN-LINKED NADP-MALATE DEHYDROGENASE FROM CORN LEAVES.

Authors:  J P Jacquot; B B Buchanan
Journal:  Plant Physiol       Date:  1981-08       Impact factor: 8.340

6.  Biosynthesis of active spinach-chloroplast thioredoxin f in transformed E. coli.

Authors:  F Aguilar; B Brunner; L Gardet-Salvi; E Stutz; P Schürmann
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

7.  Purification and characterization of pea thioredoxin f expressed in Escherichia coli.

Authors:  M Hodges; M Miginiac-Maslow; P Decottignies; J P Jacquot; M Stein; L Lepiniec; C Crétin; P Gadal
Journal:  Plant Mol Biol       Date:  1994-10       Impact factor: 4.076

8.  NADP regulates the light activation of NADP-dependent malate dehydrogenase.

Authors:  R Scheibe; J P Jacquot
Journal:  Planta       Date:  1983-05       Impact factor: 4.116

9.  Regulation of fructose-1,6-bisphosphatase activity in leaves.

Authors:  R C Leegood; D A Walker
Journal:  Planta       Date:  1982-12       Impact factor: 4.116
  9 in total

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