Literature DB >> 22493331

Quantification by real-time PCR assay of Staphylococcus aureus load: a useful tool for rapidly identifying persistent nasal carriers.

Paul O Verhoeven1, Florence Grattard, Anne Carricajo, Frédéric Lucht, Céline Cazorla, Olivier Garraud, Bruno Pozzetto, Philippe Berthelot.   

Abstract

The Cepheid Xpert MRSA/SA nasal PCR assay was compared to culture for quantifying Staphylococcus aureus load from 104 nasal samples (r = 0.91, P < 0.0001). Using a bacterial load-based algorithm, the test was found able to predict the carrier state in 32 of 35 healthy volunteers (22 persistent and 13 nonpersistent carriers).

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Year:  2012        PMID： 22493331      PMCID： PMC3372148          DOI： 10.1128/JCM.00157-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Quantitative real-time polymerase chain reaction for enteropathogenic Escherichia coli: a tool for investigation of asymptomatic versus symptomatic infections.

Authors:  Francesca Barletta; Theresa J Ochoa; Erik Mercado; Joaquim Ruiz; Lucie Ecker; Giovanni Lopez; Monica Mispireta; Ana I Gil; Claudio F Lanata; Thomas G Cleary
Journal:  Clin Infect Dis       Date:  2011-10-25       Impact factor: 9.079

2.  Evaluation of the Xpert MRSA assay for rapid detection of methicillin-resistant Staphylococcus aureus from nares swabs of geriatric hospitalized patients and failure to detect a specific SCCmec type IV variant.

Authors:  C Laurent; P Bogaerts; D Schoevaerdts; O Denis; A Deplano; C Swine; M J Struelens; Y Glupczynski
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-05-29       Impact factor: 3.267

3.  Severity of pneumococcal pneumonia associated with genomic bacterial load.

Authors:  Jordi Rello; Thiago Lisboa; Manel Lujan; Miguel Gallego; Cordelia Kee; Ian Kay; Diego Lopez; Grant W Waterer
Journal:  Chest       Date:  2009-05-11       Impact factor: 9.410

4.  Development of a real-time PCR targeting the yidC gene for the detection of Mycoplasma hominis and comparison with quantitative culture.

Authors:  C Férandon; O Peuchant; C Janis; A Benard; H Renaudin; S Pereyre; C Bébéar
Journal:  Clin Microbiol Infect       Date:  2011-02       Impact factor: 8.067

5.  An algorithm based on one or two nasal samples is accurate to identify persistent nasal carriers of Staphylococcus aureus.

Authors:  P O Verhoeven; F Grattard; A Carricajo; F Lucht; C Cazorla; O Garraud; B Pozzetto; P Berthelot
Journal:  Clin Microbiol Infect       Date:  2011-08-18       Impact factor: 8.067

Review 6.  The role of nasal carriage in Staphylococcus aureus infections.

Authors:  Heiman F L Wertheim; Damian C Melles; Margreet C Vos; Willem van Leeuwen; Alex van Belkum; Henri A Verbrugh; Jan L Nouwen
Journal:  Lancet Infect Dis       Date:  2005-12       Impact factor: 25.071

7.  Persistent (not intermittent) nasal carriage of Staphylococcus aureus is the determinant of CPD-related infections.

Authors:  Jan L Nouwen; Marien W J A Fieren; Susan Snijders; Henri A Verbrugh; Alex van Belkum
Journal:  Kidney Int       Date:  2005-03       Impact factor: 10.612

8.  Predicting the Staphylococcus aureus nasal carrier state: derivation and validation of a "culture rule".

Authors:  Jan L Nouwen; Alewijn Ott; Marjolein F Q Kluytmans-Vandenbergh; Hélène A M Boelens; Albert Hofman; Alex van Belkum; Henri A Verbrugh
Journal:  Clin Infect Dis       Date:  2004-08-26       Impact factor: 9.079

9.  Non-spa-typeable clinical Staphylococcus aureus strains are naturally occurring protein A mutants.

Authors:  Cathrin Baum; Bettina Haslinger-Löffler; Henrik Westh; Kit Boye; Georg Peters; Claudia Neumann; Barbara C Kahl
Journal:  J Clin Microbiol       Date:  2009-09-16       Impact factor: 5.948

10.  Measuring nasal bacterial load and its association with otitis media.

Authors:  Heidi Smith-Vaughan; Roy Byun; Mangala Nadkarni; Nicholas A Jacques; Neil Hunter; Stephen Halpin; Peter S Morris; Amanda J Leach
Journal:  BMC Ear Nose Throat Disord       Date:  2006-05-10
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  5 in total

1.  Rapid detection of carbapenemase activity through monitoring ertapenem hydrolysis in Enterobacteriaceae with LC-MS/MS.

Authors:  David R Peaper; Mandar V Kulkarni; Alexandra N Tichy; Michael Jarvis; Thomas S Murray; Michael E Hodsdon
Journal:  Bioanalysis       Date:  2013-01       Impact factor: 2.681

2.  Identifying Hemodialysis Patients With the Highest Risk of Staphylococcus aureus Endogenous Infection Through a Simple Nasal Sampling Algorithm.

Authors:  Paul O Verhoeven; Julie Gagnaire; Cyrille H Haddar; Florence Grattard; Damien Thibaudin; Aida Afiani; Céline Cazorla; Anne Carricajo; Christophe Mariat; Eric Alamartine; Frédéric Lucht; Olivier Garraud; Bruno Pozzetto; Elisabeth Botelho-Nevers; Philippe Berthelot
Journal:  Medicine (Baltimore)       Date:  2016-04       Impact factor: 1.889

Review 3.  Advances and Challenges in Viability Detection of Foodborne Pathogens.

Authors:  Dexin Zeng; Zi Chen; Yuan Jiang; Feng Xue; Baoguang Li
Journal:  Front Microbiol       Date:  2016-11-22       Impact factor: 5.640

4.  Direct detection of nasal Staphylococcus aureus carriage via helicase-dependent isothermal amplification and chip hybridization.

Authors:  Georges C Frech; Denton Munns; Robert D Jenison; Brian J Hicke
Journal:  BMC Res Notes       Date:  2012-08-11

Review 5.  Laboratory-Based and Point-of-Care Testing for MSSA/MRSA Detection in the Age of Whole Genome Sequencing.

Authors:  Alex van Belkum; Olivier Rochas
Journal:  Front Microbiol       Date:  2018-06-29       Impact factor: 5.640
  5 in total

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