Literature DB >> 22493328

Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry.

Narasimha V Hegde1, Bhushan M Jayarao, Chitrita DebRoy.   

Abstract

Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10(3) target cells in a bacterial mixture of 10(5) CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

Entities:  

Mesh：

Year:  2012        PMID： 22493328      PMCID： PMC3372132          DOI： 10.1128/JCM.00137-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

Review 1.  Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting.

Authors:  G Nebe-von-Caron; P J Stephens; C J Hewitt; J R Powell; R A Badley
Journal:  J Microbiol Methods       Date:  2000-09       Impact factor: 2.363

Review 2.  Microbial analysis at the single-cell level: tasks and techniques.

Authors:  H M Shapiro
Journal:  J Microbiol Methods       Date:  2000-09       Impact factor: 2.363

3.  Quantitative isolation efficiency of O26, O103, O111, O145 and O157 STEC serotypes from artificially contaminated food and cattle faeces samples using a new isolation protocol.

Authors:  B Possé; L De Zutter; M Heyndrickx; L Herman
Journal:  J Appl Microbiol       Date:  2008-02-14       Impact factor: 3.772

4.  Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow cytometer.

Authors:  Lingling Yang; Lina Wu; Shaobin Zhu; Yao Long; Wei Hang; Xiaomei Yan
Journal:  Anal Chem       Date:  2010-02-01       Impact factor: 6.986

5.  Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111, and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef.

Authors:  Angela M Valadez; Chitrita Debroy; Edward Dudley; Catherine N Cutter
Journal:  J Food Prot       Date:  2011-02       Impact factor: 2.077

Review 6.  Detection of O antigens in Escherichia coli.

Authors:  Chitrita DebRoy; Elisabeth Roberts; Pina M Fratamico
Journal:  Anim Health Res Rev       Date:  2011-12       Impact factor: 2.615

7.  Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef.

Authors:  Pina M Fratamico; Lori K Bagi; William C Cray; Neelam Narang; Xianghe Yan; Marjorie Medina; Yanhong Liu
Journal:  Foodborne Pathog Dis       Date:  2011-01-09       Impact factor: 3.171

8.  Detection of Shiga toxin-producing Escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster.

Authors:  Chitrita DebRoy; Elisabeth Roberts; Angela M Valadez; Edward G Dudley; Catherine N Cutter
Journal:  Foodborne Pathog Dis       Date:  2011-05       Impact factor: 3.171

9.  Recovery of GFP-labeled bacteria for culturing and molecular analysis after cell sorting using a benchtop flow cytometer.

Authors:  B C Ferrari; G Oregaard; S J Sørensen
Journal:  Microb Ecol       Date:  2004-05-27       Impact factor: 4.552
  9 in total
  3 in total

Review 1.  A review on detection methods used for foodborne pathogens.

Authors:  B Priyanka; Rajashekhar K Patil; Sulatha Dwarakanath
Journal:  Indian J Med Res       Date:  2016-09       Impact factor: 2.375

2.  Comparison of O-Antigen Gene Clusters of All O-Serogroups of Escherichia coli and Proposal for Adopting a New Nomenclature for O-Typing.

Authors:  Chitrita DebRoy; Pina M Fratamico; Xianghe Yan; GianMarco Baranzoni; Yanhong Liu; David S Needleman; Robert Tebbs; Catherine D O'Connell; Adam Allred; Michelle Swimley; Michael Mwangi; Vivek Kapur; Juan A Raygoza Garay; Elisabeth L Roberts; Robab Katani
Journal:  PLoS One       Date:  2016-01-29       Impact factor: 3.240

3.  A recombinant O-polysaccharide-protein conjugate approach to develop highly specific monoclonal antibodies to Shiga toxin-producing Escherichia coli O157 and O145 serogroups.

Authors:  Daniela S Castillo; Diego A Rey Serantes; Luciano J Melli; Andrés E Ciocchini; Juan E Ugalde; Diego J Comerci; Alejandro Cassola
Journal:  PLoS One       Date:  2017-10-05       Impact factor: 3.240
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.