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Literature DB >> 22492441

Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.

Erica M Hartmann¹, Jonathan P Badalamenti, Rosa Krajmalnik-Brown, Rolf U Halden.

Abstract

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.

Entities: Chemical Species

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Year: 2012 PMID： 22492441 PMCID： PMC3370553 DOI： 10.1128/AEM.00715-12

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

24 in total

1. How quantitative is quantitative PCR with respect to cell counts?

Authors: W Ludwig; K H Schleifer
Journal: Syst Appl Microbiol Date: 2000-12 Impact factor: 4.022

2. Occurrence and loss over three years of 72 pharmaceuticals and personal care products from biosolids-soil mixtures in outdoor mesocosms.

Authors: Evelyn Walters; Kristin McClellan; Rolf U Halden
Journal: Water Res Date: 2010-07-27 Impact factor: 11.236

Review 3. The genus Sphingomonas: physiology and ecology.

Authors: D C White; S D Sutton; D B Ringelberg
Journal: Curr Opin Biotechnol Date: 1996-06 Impact factor: 9.740

Review 4. Molecular bases of aerobic bacterial degradation of dioxins: involvement of angular dioxygenation.

Authors: Hideaki Nojiri; Toshio Omori
Journal: Biosci Biotechnol Biochem Date: 2002-10 Impact factor: 2.043

5. The lin genes for γ-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids.

Authors: Michiro Tabata; Ryo Endo; Michihiro Ito; Yoshiyuki Ohtsubo; Ashwani Kumar; Masataka Tsuda; Yuji Nagata
Journal: Biosci Biotechnol Biochem Date: 2011-03-07 Impact factor: 2.043

6. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria.

Authors: Hiroshi Maeda; Chiyo Fujimoto; Yasuhiro Haruki; Takemasa Maeda; Susumu Kokeguchi; Millan Petelin; Hideo Arai; Ichiro Tanimoto; Fusanori Nishimura; Shogo Takashiba
Journal: FEMS Immunol Med Microbiol Date: 2003-10-24

Review 7. Dioxin risks in perspective: past, present, and future.

Authors: Sean M Hays; Lesa L Aylward
Journal: Regul Toxicol Pharmacol Date: 2003-04 Impact factor: 3.271

8. A real-time polymerase chain reaction method for monitoring anaerobic, hydrocarbon-degrading bacteria based on a catabolic gene.

Authors: Harry R Beller; Staci R Kane; Tina C Legler; Pedro J J Alvarez
Journal: Environ Sci Technol Date: 2002-09-15 Impact factor: 9.028

Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.

1. How quantitative is quantitative PCR with respect to cell counts?

2. Occurrence and loss over three years of 72 pharmaceuticals and personal care products from biosolids-soil mixtures in outdoor mesocosms.

Review 3. The genus Sphingomonas: physiology and ecology.

Review 4. Molecular bases of aerobic bacterial degradation of dioxins: involvement of angular dioxygenation.

5. The lin genes for γ-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids.

6. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria.

Review 7. Dioxin risks in perspective: past, present, and future.

8. A real-time polymerase chain reaction method for monitoring anaerobic, hydrocarbon-degrading bacteria based on a catabolic gene.

9. Detection and characterization of conjugative degradative plasmids in xenobiotic-degrading Sphingomonas strains.

10. Biotransformation of 2,7-dichloro- and 1,2,3,4-tetrachlorodibenzo-p-dioxin by Sphingomonas wittichii RW1.

Review 1. The Divided Bacterial Genome: Structure, Function, and Evolution.

2. Proteomic profiling of the dioxin-degrading bacterium Sphingomonas wittichii RW1.

3. Functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter.