Literature DB >> 22485991

Detection of ubiquitylated proteins in mammalian cells.

William P Tansey1.   

Abstract

This assay is performed to detect ubiquitylated proteins in transfected mammalian cells. Twenty-four to 40 hours after transfection, cells are harvested, total ubiquitylated proteins are recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.

Entities:  

Year:  2006        PMID： 22485991     DOI： 10.1101/pdb.prot4616

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  4 in total

1.  The DNA damage response induces IFN.

Authors:  Sabrina Brzostek-Racine; Chris Gordon; Sarah Van Scoy; Nancy C Reich
Journal:  J Immunol       Date:  2011-10-17       Impact factor: 5.422

2.  USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.

Authors:  Ji Li; Vincenzo D'Angiolella; E Scott Seeley; Sehyun Kim; Tetsuo Kobayashi; Wenxiang Fu; Eric I Campos; Michele Pagano; Brian David Dynlacht
Journal:  Nature       Date:  2013-03-14       Impact factor: 49.962

3.  Nuclear import of SAMHD1 is mediated by a classical karyopherin α/β1 dependent pathway and confers sensitivity to VpxMAC induced ubiquitination and proteasomal degradation.

Authors:  Torsten Schaller; Darja Pollpeter; Luis Apolonia; Caroline Goujon; Michael H Malim
Journal:  Retrovirology       Date:  2014-04-08       Impact factor: 4.602

4.  Interplay between protein acetylation and ubiquitination controls MCL1 protein stability.

Authors:  Kouhei Shimizu; Min Gi; Shugo Suzuki; Brian J North; Asami Watahiki; Satoshi Fukumoto; John M Asara; Fuminori Tokunaga; Wenyi Wei; Hiroyuki Inuzuka
Journal:  Cell Rep       Date:  2021-11-09       Impact factor: 9.423
  4 in total

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