Literature DB >> 22485895

Isolation and purification of tetrahymena nuclei.

Melody T Sweet, C David Allis.   

Abstract

INTRODUCTIONThe procedures described below allow the isolation of nuclei from all stages of the Tetrahymena life cycle in high yield with a high degree of purity. This method gives highly purified populations of both micronuclei and macronuclei.

Year:  2006        PMID： 22485895     DOI： 10.1101/pdb.prot4500

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  5 in total

1.  Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena.

Authors:  Ursula E Schoeberl; Henriette M Kurth; Tomoko Noto; Kazufumi Mochizuki
Journal:  Genes Dev       Date:  2012-08-01       Impact factor: 11.361

2.  A Polycomb repressive complex is required for RNAi-mediated heterochromatin formation and dynamic distribution of nuclear bodies.

Authors:  Jing Xu; Xiaolu Zhao; Fengbiao Mao; Venkatesha Basrur; Beatrix Ueberheide; Brian T Chait; C David Allis; Sean D Taverna; Shan Gao; Wei Wang; Yifan Liu
Journal:  Nucleic Acids Res       Date:  2021-06-04       Impact factor: 16.971

3.  Dissecting relative contributions of cis- and trans-determinants to nucleosome distribution by comparing Tetrahymena macronuclear and micronuclear chromatin.

Authors:  Jie Xiong; Shan Gao; Wen Dui; Wentao Yang; Xiao Chen; Sean D Taverna; Ronald E Pearlman; Wendy Ashlock; Wei Miao; Yifan Liu
Journal:  Nucleic Acids Res       Date:  2016-08-03       Impact factor: 16.971

4.  A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.

Authors:  Lifang Feng; Guangying Wang; Eileen P Hamilton; Jie Xiong; Guanxiong Yan; Kai Chen; Xiao Chen; Wen Dui; Amber Plemens; Lara Khadr; Arjune Dhanekula; Mina Juma; Hung Quang Dang; Geoffrey M Kapler; Eduardo Orias; Wei Miao; Yifan Liu
Journal:  Nucleic Acids Res       Date:  2017-09-19       Impact factor: 16.971

5.  Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis.

Authors:  Romeo Papazyan; Ekaterina Voronina; Jessica R Chapman; Teresa R Luperchio; Tonya M Gilbert; Elizabeth Meier; Samuel G Mackintosh; Jeffrey Shabanowitz; Alan J Tackett; Karen L Reddy; Robert S Coyne; Donald F Hunt; Yifan Liu; Sean D Taverna
Journal:  Elife       Date:  2014-08-26       Impact factor: 8.140
  5 in total

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