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Literature DB >> 22484684

In planta transformation of Arabidopsis.

Abstract

INTRODUCTIONA breakthrough in Arabidopsis research was the invention of the vacuum-infiltration procedure, a simple and reliable method of obtaining transformants at high efficiency while avoiding the use of tissue culture. The plant transformation procedures described here involve floral dip, vacuum infiltration, and spraying. They yield transformants at frequencies ranging up to several percent, with the most common frequency being 0.1%-1%.

Entities: Species

Year: 2006 PMID： 22484684 DOI： 10.1101/pdb.prot4668

Source DB: PubMed Journal: CSH Protoc ISSN： 1559-6095

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Cited

16 in total

1. POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility.

Authors: Adam Jozwiak; Malgorzata Gutkowska; Katarzyna Gawarecka; Liliana Surmacz; Anna Buczkowska; Malgorzata Lichocka; Julita Nowakowska; Ewa Swiezewska
Journal: Plant Cell Date: 2015-12-01 Impact factor: 11.277

2. Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana.

Authors: V C Beracochea; N I Almasia; L Peluffo; V Nahirñak; E H Hopp; N Paniego; R A Heinz; C Vazquez-Rovere; V V Lia
Journal: Plant Cell Rep Date: 2015-06-13 Impact factor: 4.570

3. AXY8 encodes an α-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides.

Authors: Markus Günl; Lutz Neumetzler; Florian Kraemer; Amancio de Souza; Alex Schultink; Maria Pena; William S York; Markus Pauly
Journal: Plant Cell Date: 2011-11-11 Impact factor: 11.277

4. Genome-wide association study using cellular traits identifies a new regulator of root development in Arabidopsis.

Authors: Mónica Meijón; Santosh B Satbhai; Takashi Tsuchimatsu; Wolfgang Busch
Journal: Nat Genet Date: 2013-11-10 Impact factor: 38.330

5. MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage.

Authors: Cătălin Voiniciuc; Maximilian Heinrich-Wilhelm Schmidt; Adeline Berger; Bo Yang; Berit Ebert; Henrik V Scheller; Helen M North; Björn Usadel; Markus Günl
Journal: Plant Physiol Date: 2015-07-28 Impact factor: 8.340

In planta transformation of Arabidopsis.

1. POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility.

2. Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana.

3. AXY8 encodes an α-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides.

4. Genome-wide association study using cellular traits identifies a new regulator of root development in Arabidopsis.

5. MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage.

6. A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis.

7. Identification of Key Enzymes for Pectin Synthesis in Seed Mucilage.

8. Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.

9. HYL1 controls the miR156-mediated juvenile phase of vegetative growth.

10. Extensive Natural Variation in Arabidopsis Seed Mucilage Structure.