Literature DB >> 22484682

Transformation of agrobacterium using the freeze-thaw method.

Detlef Weigel, Jane Glazebrook.   

Abstract

INTRODUCTIONThis protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.

Entities:  

Year:  2006        PMID： 22484682     DOI： 10.1101/pdb.prot4666

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  84 in total

1.  An assessment of transgenomics as a tool for identifying genes involved in the evolutionary differentiation of closely related plant species.

Authors:  Raul Correa; John Stanga; Bret Larget; Aaron Roznowski; Guoping Shu; Brian Dilkes; David A Baum
Journal:  New Phytol       Date:  2011-11-11       Impact factor: 10.151

2.  Functional characterization of a Chrysanthemum dichrum stress-related promoter.

Authors:  Yu Chen; Sumei Chen; Fadi Chen; Pirui Li; Lin Chen; Zhiyong Guan; Qingshan Chang
Journal:  Mol Biotechnol       Date:  2012-10       Impact factor: 2.695

3.  Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker.

Authors:  Raham Sher Khan; Valentine Otang Ntui; Dong Poh Chin; Ikuo Nakamura; Masahiro Mii
Journal:  Plant Cell Rep       Date:  2010-12-24       Impact factor: 4.570

4.  Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection.

Authors:  Hye-Young Lee; So Eui Lee; Jongchan Woo; Doil Choi; Eunsook Park
Journal:  J Vis Exp       Date:  2018-05-24       Impact factor: 1.355

5.  Development of rapid microwave-mediated and low-temperature bacterial transformations.

Authors:  Valerie T Tripp; Johnathan C Maza; Douglas D Young
Journal:  J Chem Biol       Date:  2013-05-21

6.  Xerophyta viscosa Aldose Reductase, XvAld1, Enhances Drought Tolerance in Transgenic Sweetpotato.

Authors:  Wilton Mbinda; Omwoyo Ombori; Christina Dixelius; Richard Oduor
Journal:  Mol Biotechnol       Date:  2018-03       Impact factor: 2.695

7.  POLYAMINE OXIDASE 1 from rice (Oryza sativa) is a functional ortholog of Arabidopsis POLYAMINE OXIDASE 5.

Authors:  Taibo Liu; Dong Wook Kim; Masaru Niitsu; Thomas Berberich; Tomonobu Kusano
Journal:  Plant Signal Behav       Date:  2014

8.  The APSES transcription factor Vst1 is a key regulator of development in microsclerotium- and resting mycelium-producing Verticillium species.

Authors:  Jorge L Sarmiento-Villamil; Nicolás E García-Pedrajas; Lourdes Baeza-Montañez; María D García-Pedrajas
Journal:  Mol Plant Pathol       Date:  2017-01-13       Impact factor: 5.663

9.  ShNPSN11, a vesicle-transport-related gene, confers disease resistance in tomato to Oidium neolycopersici.

Authors:  Qinggui Lian; Yanan Meng; Xinbei Zhao; Yuanliu Xu; Yang Wang; Brad Day; Qing Ma
Journal:  Biochem J       Date:  2020-10-16       Impact factor: 3.857

10.  Characterization of Arabidopsis lines deficient in GAPC-1, a cytosolic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase.

Authors:  Sebastián P Rius; Paula Casati; Alberto A Iglesias; Diego F Gomez-Casati
Journal:  Plant Physiol       Date:  2008-09-26       Impact factor: 8.340
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.