Literature DB >> 22484681

Transformation of agrobacterium using electroporation.

Detlef Weigel, Jane Glazebrook.   

Abstract

INTRODUCTIONThis protocol describes a method for transforming Agrobacterium with plasmid DNA using electroporation in a manner similar to that commonly used for Escherichia coli. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of Agrobacterium transformants with this technique.

Entities:  

Year:  2006        PMID： 22484681     DOI： 10.1101/pdb.prot4665

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  16 in total

1.  A genomic-scale artificial microRNA library as a tool to investigate the functionally redundant gene space in Arabidopsis.

Authors:  Felix Hauser; Wenxiao Chen; Ulrich Deinlein; Kenneth Chang; Stephan Ossowski; Joffrey Fitz; Gregory J Hannon; Julian I Schroeder
Journal:  Plant Cell       Date:  2013-08-16       Impact factor: 11.277

2.  Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in Arabidopsis thaliana.

Authors:  Sankalpi N Warnasooriya; Katie J Porter; Beronda L Montgomery
Journal:  Plant Signal Behav       Date:  2011-05-01

3.  Peptide macrocyclization catalyzed by a prolyl oligopeptidase involved in α-amanitin biosynthesis.

Authors:  Hong Luo; Sung-Yong Hong; R Michael Sgambelluri; Evan Angelos; Xuan Li; Jonathan D Walton
Journal:  Chem Biol       Date:  2014-12-04

4.  Overexpression of AlTMP2 gene from the halophyte grass Aeluropus littoralis in transgenic tobacco enhances tolerance to different abiotic stresses by improving membrane stability and deregulating some stress-related genes.

Authors:  Walid Ben-Romdhane; Rania Ben-Saad; Donaldo Meynard; Nabil Zouari; Ali Mahjoub; Lotfi Fki; Emmanuel Guiderdoni; Abdullah Al-Doss; Afif Hassairi
Journal:  Protoplasma       Date:  2018-02-15       Impact factor: 3.356

5.  Sulfur deficiency-induced repressor proteins optimize glucosinolate biosynthesis in plants.

Authors:  Fayezeh Aarabi; Miyuki Kusajima; Takayuki Tohge; Tomokazu Konishi; Tamara Gigolashvili; Makiko Takamune; Yoko Sasazaki; Mutsumi Watanabe; Hideo Nakashita; Alisdair R Fernie; Kazuki Saito; Hideki Takahashi; Hans-Michael Hubberten; Rainer Hoefgen; Akiko Maruyama-Nakashita
Journal:  Sci Adv       Date:  2016-10-07       Impact factor: 14.136

6.  Ectopic Expression of Aeluropus littoralis Plasma Membrane Protein Gene AlTMP1 Confers Abiotic Stress Tolerance in Transgenic Tobacco by Improving Water Status and Cation Homeostasis.

Authors:  Walid Ben Romdhane; Rania Ben-Saad; Donaldo Meynard; Jean-Luc Verdeil; Jalel Azaza; Nabil Zouari; Lotfi Fki; Emmanuel Guiderdoni; Abdullah Al-Doss; Afif Hassairi
Journal:  Int J Mol Sci       Date:  2017-03-24       Impact factor: 5.923

7.  Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus.

Authors:  Xue Chen; Guanqun Chen; Martin Truksa; Crystal L Snyder; Saleh Shah; Randall J Weselake
Journal:  J Exp Bot       Date:  2014-05-12       Impact factor: 6.992

8.  Interaction between Nitrogen and Phosphate Stress Responses in Sinorhizobium meliloti.

Authors:  Kelly L Hagberg; Svetlana N Yurgel; Monika Mulder; Michael L Kahn
Journal:  Front Microbiol       Date:  2016-11-30       Impact factor: 5.640

9.  Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere.

Authors:  Henk J Schouten; Henri Vande Geest; Sofia Papadimitriou; Marian Bemer; Jan G Schaart; Marinus J M Smulders; Gabino Sanchez Perez; Elio Schijlen
Journal:  Plant Cell Rep       Date:  2017-02-02       Impact factor: 4.570

10.  Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology.

Authors:  Edgar M Medina; Kristyn A Robinson; Kimberly Bellingham-Johnstun; Giuseppe Ianiri; Caroline Laplante; Lillian K Fritz-Laylin; Nicolas E Buchler
Journal:  Elife       Date:  2020-05-11       Impact factor: 8.140
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