Literature DB >> 22484670

Freeze-thaw lysis for extraction of proteins from Mammalian cells.

William P Tansey1.   

Abstract

INTRODUCTIONThis protocol describes the extraction of proteins from mammalian cells grown in 6- or 10-cm tissue-culture dishes. It is useful for making very concentrated extracts for Western blotting, and is particularly helpful when low-abundance proteins such as Myc are being studied.

Entities:  

Year:  2006        PMID： 22484670     DOI： 10.1101/pdb.prot4614

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  8 in total

1.  Heterogeneous Mechanisms of Secondary Resistance and Clonal Selection in Sarcoma during Treatment with Nutlin.

Authors:  Audrey Laroche; Kevin Tran-Cong; Vanessa Chaire; Pauline Lagarde; Isabelle Hostein; Jean-Michel Coindre; Frederic Chibon; Agnes Neuville; Tom Lesluyes; Carlo Lucchesi; Antoine Italiano
Journal:  PLoS One       Date:  2015-10-01       Impact factor: 3.240

2.  Canine adipose-derived stromal cell viability following exposure to synovial fluid from osteoarthritic joints.

Authors:  Kristina M Kiefer; Timothy D O'Brien; Elizabeth G Pluhar; Michael Conzemius
Journal:  Vet Rec Open       Date:  2015-07-24

3.  PRIMA-1(MET) induces death in soft-tissue sarcomas cell independent of p53.

Authors:  Thomas Grellety; Audrey Laroche-Clary; Vanessa Chaire; Pauline Lagarde; Frédéric Chibon; Agnes Neuville; Antoine Italiano
Journal:  BMC Cancer       Date:  2015-10-13       Impact factor: 4.430

4.  α-Catenin homodimers are recruited to phosphoinositide-activated membranes to promote adhesion.

Authors:  Megan N Wood; Noboru Ishiyama; Indira Singaram; Connie M Chung; Annette S Flozak; Alex Yemelyanov; Mitsu Ikura; Wonhwa Cho; Cara J Gottardi
Journal:  J Cell Biol       Date:  2017-09-05       Impact factor: 10.539

5.  Combined targeting of MDM2 and CDK4 is synergistic in dedifferentiated liposarcomas.

Authors:  Audrey Laroche-Clary; Vanessa Chaire; Marie-Paule Algeo; Marie-Alix Derieppe; François L Loarer; Antoine Italiano
Journal:  J Hematol Oncol       Date:  2017-06-19       Impact factor: 17.388

6.  Screening of chemical library against whole cell kinome activity via non-radioactive, high throughput kinase assay.

Authors:  Ravi Jain; Swati Garg; Shailja Singh
Journal:  MethodsX       Date:  2019-01-18

7.  CTL-Derived Exosomes Enhance the Activation of CTLs Stimulated by Low-Affinity Peptides.

Authors:  Shu-Wei Wu; Lei Li; Yan Wang; Zhengguo Xiao
Journal:  Front Immunol       Date:  2019-06-04       Impact factor: 7.561

8.  Protein Arginine Methyltransferase 5 as a Therapeutic Target for KRAS Mutated Colorectal Cancer.

Authors:  David Shifteh; Tzuriel Sapir; Moshe Pahmer; Adam Haimowitz; Sanjay Goel; Radhashree Maitra
Journal:  Cancers (Basel)       Date:  2020-07-28       Impact factor: 6.639
  8 in total

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