BACKGROUND: Adipose tissue-derived mesenchymal stem cells (ADSCs) are an attractive source for regenerative medicine because they are easily accessible through minimally invasive methods. We investigated the efficacy of ADSC transplantation on outcome after hepatic ischemia-reperfusion and subsequent hepatectomy in rats. METHODS: ADSCs were isolated from subcutaneous adipose tissue of rats. After clamping the hepatoduodenal ligament for 15 min, the rats were subjected to a 70% partial hepatectomy. After releasing the clamp, 2 × 10(6) ADSCs per rat were injected through the penile vein. Phosphate buffered saline was injected as a control. The parameters of hepatic regeneration, such as hepatic regeneration rate, mitotic index, and anti-proliferating cell nuclear antigen levels, were examined. Furthermore, the expression of hepatic regeneration-associated proteins and genes in the regenerating liver was determined. RESULTS: The hepatic regeneration rate 2 d after hepatectomy was significantly greater in the ADSC transplanted group compared with the sham group. Mitotic index, anti-proliferating cell nuclear antigen levels, and other regeneration-associated proteins in the liver were significantly higher in the ADSC transplanted group than the sham group on 1 d after hepatectomy. A number of hepatic regeneration-associated genes also were significantly upregulated in the ADSC transplanted group. CONCLUSIONS: These results indicate that ADSC transplantation may provide beneficial effects in the process of liver regeneration after hepatic ischemia-reperfusion and subsequent hepatectomy.
BACKGROUND: Adipose tissue-derived mesenchymal stem cells (ADSCs) are an attractive source for regenerative medicine because they are easily accessible through minimally invasive methods. We investigated the efficacy of ADSC transplantation on outcome after hepatic ischemia-reperfusion and subsequent hepatectomy in rats. METHODS: ADSCs were isolated from subcutaneous adipose tissue of rats. After clamping the hepatoduodenal ligament for 15 min, the rats were subjected to a 70% partial hepatectomy. After releasing the clamp, 2 × 10(6) ADSCs per rat were injected through the penile vein. Phosphate buffered saline was injected as a control. The parameters of hepatic regeneration, such as hepatic regeneration rate, mitotic index, and anti-proliferating cell nuclear antigen levels, were examined. Furthermore, the expression of hepatic regeneration-associated proteins and genes in the regenerating liver was determined. RESULTS: The hepatic regeneration rate 2 d after hepatectomy was significantly greater in the ADSC transplanted group compared with the sham group. Mitotic index, anti-proliferating cell nuclear antigen levels, and other regeneration-associated proteins in the liver were significantly higher in the ADSC transplanted group than the sham group on 1 d after hepatectomy. A number of hepatic regeneration-associated genes also were significantly upregulated in the ADSC transplanted group. CONCLUSIONS: These results indicate that ADSC transplantation may provide beneficial effects in the process of liver regeneration after hepatic ischemia-reperfusion and subsequent hepatectomy.
Authors: Gregory T Everson; John C Hoefs; Claus U Niemann; Kim M Olthoff; Robert Dupuis; Shannon Lauriski; Andrea Herman; Norah Milne; Brenda W Gillespie; Nathan P Goodrich; James E Everhart Journal: Liver Transpl Date: 2013-03 Impact factor: 5.799
Authors: Eva Koellensperger; Willem Niesen; Jonas Kolbenschlag; Felix Gramley; Guenter Germann; Uwe Leimer Journal: Stem Cells Int Date: 2013-11-07 Impact factor: 5.443
Authors: Suomi M G Fouraschen; Joshua H Wolf; Luc J W van der Laan; Petra E de Ruiter; Wayne W Hancock; Job P van Kooten; Monique M A Verstegen; Kim M Olthoff; Jeroen de Jonge Journal: J Immunol Res Date: 2015-08-24 Impact factor: 4.818
Authors: Nele Boeykens; Peter Ponsaerts; Annemie Van der Linden; Zwi Berneman; Dirk Ysebaert; Kathleen De Greef Journal: PLoS One Date: 2013-07-18 Impact factor: 3.240