AIM: To investigate differently expressed genes associated with cardiac fibrosis induced independently by aldosterone. METHODS: Fetal cardiac fibroblasts (FCFs)were isolated and cultured. Total RNA was extracted 8 hours after aldosterone administration. Then gene chips were used to screen these RNA samples. Some of candidate genes were confirmed by RT-PCR and Western blot. RESULTS: Differently expressed 1519 genes were screened. Up-regulated genes were 714 while down-regulated genes were 805. The expression of CCL7, MMP-26 and IL31RA was tested by RT-PCR and western blot, the results is identical with those by gene chips. CONCLUSION: Gene chip can efficiently single out differently expressed genes induced dependently by aldosterone in FCFs. CCL7, MMP-26 and IL31RA may be associated with cardiac fibrosis induced by aldosterone.
AIM: To investigate differently expressed genes associated with cardiac fibrosis induced independently by aldosterone. METHODS: Fetal cardiac fibroblasts (FCFs)were isolated and cultured. Total RNA was extracted 8 hours after aldosterone administration. Then gene chips were used to screen these RNA samples. Some of candidate genes were confirmed by RT-PCR and Western blot. RESULTS: Differently expressed 1519 genes were screened. Up-regulated genes were 714 while down-regulated genes were 805. The expression of CCL7, MMP-26 and IL31RA was tested by RT-PCR and western blot, the results is identical with those by gene chips. CONCLUSION: Gene chip can efficiently single out differently expressed genes induced dependently by aldosterone in FCFs. CCL7, MMP-26 and IL31RA may be associated with cardiac fibrosis induced by aldosterone.
Authors: D Q Shih; L Zheng; X Zhang; H Zhang; Y Kanazawa; R Ichikawa; K L Wallace; J Chen; C Pothoulakis; H W Koon; S R Targan Journal: Mucosal Immunol Date: 2014-05-21 Impact factor: 7.313