A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity.

Many animals possess neurons specialized for the detection of carbon dioxide (CO(2)), which acts as a cue to elicit behavioral responses and is also an internally generated product of respiration that regulates animal physiology. In many organisms how such neurons detect CO(2) is poorly understood. We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2). The ETS-5 transcription factor is necessary for the specification of CO(2)-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient to bypass a requirement for ets-5 in CO(2)-detection and transforms neurons into CO(2)-sensing neurons. Because ETS-5 and GCY-9 are members of gene families that are conserved between nematodes and vertebrates, a similar mechanism might act in the specification of CO(2)-sensing neurons in other phyla.

Introduction

CO2-chemosensitive neurons are found in many animals. In vertebrates, CO2-sensing neurons are critical regulators of respiration [1]. Their dysfunction is proposed to underlie disorders such as sudden infant death syndrome [2] and congenital hypoventilation syndrome [3]. CO2 is also sensed by animals as an ethologically relevant environmental cue. For example, insects detect CO2 in the contexts of host- and mate-finding and as an aversive odorant [4], [5], and the rodent olfactory system contains neurons that can be activated by low concentrations of CO2 [6], [7]. Studies of the insect olfactory system have identified odorant receptors that mediate CO2 sensation, indicating that CO2 can act through cellular and molecular systems dedicated to its detection [4], [8], [9]. The molecular mechanisms that mediate CO2 sensing by insect olfactory neurons are, however, unique to insects. How neurons of other organisms detect CO2 is poorly understood.

To control internal concentrations of respiratory gases, the microscopic nematode C. elegans navigates to environments with preferred concentrations of oxygen and CO2 [10], [11], [12], [13], [14], [15]. Two anterior sensory neurons, the BAG neurons, detect environmental CO2 and mediate a CO2-avoidance behavior [15], [16], [17]. CO2-sensing by BAG neurons requires cyclic nucleotide signaling; mutants that lack either TAX-2 or TAX-4 subunits of a cyclic nucleotide-gated ion channel are defective in behavioral and physiological responses to CO2 [16], [17], as are mutants that lack the receptor-type guanylate cyclase GCY-9 [17]. Because CO2 activates the BAG neurons of C. elegans through a specific molecular pathway, their study offers the opportunity to understand the molecular basis of neuronal CO2-sensing.

ETS-5, an ETS-domain-containing transcription factor, was recently shown to regulate expression of many BAG-neuron-specific genes [18]. Whether ETS-5 is required for BAG-neuron responses to CO2, and, if so, how ETS-5 confers CO2-chemosensitivity to BAG neurons is unknown. We show here that ets-5 mutants have defects in CO2 sensory transduction, and that ETS-5 directly interacts in vitro and in vivo with elements in the promoter of gcy-9, which encodes a critical component of the CO2 sensory transduction pathway. Strikingly, the requirement for ETS-5 in CO2 sensing can be bypassed by forced expression of gcy-9. These data indicate that a mechanism by which ETS-5 specifies CO2 chemosensitivity of BAG neurons is by direct regulation of the expression of GCY-9. GCY-9 in turn is sufficient to mediate neuronal responses to CO2 and likely encodes a receptor for CO2 or a CO2 metabolite.

Results

One of ten ETS-family transcription factors encoded by the C. elegans genome is required for the specification of CO2-sensing BAG neurons

To identify factors that specify BAG neurons, we analyzed promoters of genes that are expressed by BAG neurons. We hypothesized that identification of cis-regulatory elements required for expression of these terminal differentiation genes in BAG neurons would enable us to identify trans-acting factors required for BAG neuron differentiation. First we looked at the promoter of the BAG-neuron-specific neuropeptide gene flp-17. We dissected a 1460 bp flp-17 promoter and found that a 138 bp promoter fragment was sufficient to drive expression in BAG neurons (Fig. S1). We noted that this minimal promoter contains three copies of a sequence motif predicted to bind ETS-family transcription factors. We then tested whether a promoter containing a single ETS-binding site is sufficient to drive gene expression in the BAG neurons. We observed that a 31 bp sequence containing a single ETS-binding site drove expression of a reporter transgene specifically in BAG neurons (Fig. 1A). These data indicated that one or more ETS-family transcription factors function to control BAG-cell fate specification.

Figure 1

An ETS-family transcription factor is required for the specification of C. elegans CO2-chemosensitive BAG neurons.

(A) A 31 basepair DNA element comprising a single ETS-binding motif (top) drives expression of GFP specifically in the BAG chemosensitive neurons (bottom). (B) One of ten ETS-family transcription factors encoded by the C. elegans genome is required for specification of BAG neurons. Shown is percent of animals mutant for each of ten ETS-family transcription factors encoded by the C. elegans genome that are BAGL/R ON (green circles) and BAGL/R OFF (open circles) for expression of a Prom reporter transgene. N = number of animals scored. # We found one lin-1(e1777) mutant in which Prom was not expressed in BAGR. (C) Fluorescence micrographs of Prom expression in a wild-type animal, an ets-5 mutant and an ets-5 mutant carrying a wild-type copy of the ets-5 locus in a fosmid-derived transgene. BAGL/R neuron positions are marked by red circles and cells previously identified as AWAL/R [19] are marked by blue circles. The nerve ring is indicated by an arrowhead. The scale bar in lower panel is 20 µm. A: anterior, L: left. The ets-5 mutant allele was tm1734. The Prom transgene was ynIs34 and the ets-5 rescuing transgene was rpEx246.

Which of the ten ETS-family transcription factors encoded by the C. elegans genome regulates expression of BAG-neuron genes? We systematically analyzed the cell fate of CO2-chemosensitive BAG neurons in mutants for each ETS transcription factor. In wild-type animals, Prom is expressed by the two BAG neurons, and also by five other paired neurons [19] (Fig. 1 B,C). We found that nine ETS-gene mutants expressed the Prom reporter in BAG neurons. ets-5 mutants, however, failed to express Prom in BAG neurons (Fig. 1B,C). Two independent ets-5 alleles caused the same defect in BAG neuron expression, and a fosmid containing the genomic ets-5 locus complemented this defect. (Fig. 1C and Fig. S2).

These results confirm the recently reported role for ETS-5 in BAG neuron development [18]. We noted, however, an additional role for ETS-5 in the specification of AWA sensory neurons, which failed to express a Prom reporter transgene in ets-5 mutants (Fig. 1B,C). A previous report indicated that ets-5 is specifically expressed by the BAG neurons and suggested that ETS-5 functions only to specify BAG neurons but not other sensory neurons [18]. We determined the gene structure of ets-5 by cloning an ets-5 cDNA (Fig. S3), and we made an ets-5::gfp translational reporter transgene by inserting GFP coding sequences into the last exon of the ets-5 genomic locus. We observed consistent expression of ETS-5::GFP in the nuclei of BAG neurons and fourteen other neuronal nuclei, including amphid neurons likely to be AWAs (Fig. 2). ETS-5 is therefore required for specification of BAG neurons, but neither its expression nor its function is restricted to BAG neurons.

Figure 2

ETS-5 is expressed by a subset of head neurons, including the chemosensitive BAG neurons.

Ventral view of an adult hermaphrodite expressing an ets-5::gfp translational reporter transgene. BAG neurons, which express a Prom reporter, are indicated with arrows. Amphid neurons likely to be AWA neurons are indicated by arrowheads. A: anterior, R: right, the scale bar in lower panel is 20 µm. The ets-5::gfp transgene is wzIs80 and the Prom transgene is wzEx36.

ETS-5 is required for sensory transduction in CO2-sensing BAG neurons

ets-5 mutants are defective in an acute CO2 avoidance behavior [18], which we also observed (Fig. 3A). We monitored the locomotory behavior of animals exposed to a plume CO2-enriched artificial atmosphere and measured CO2-evoked reversals. The frequency of reversals by wild-type animals increased dramatically upon presentation of a CO2 stimulus (Fig. 3A). By contrast, the reversal frequency of animals carrying a caspase-expression transgene that specifically kills BAG neurons did not significantly change in response to presentation of a CO2 stimulus (Fig. 3A.) Like BAG-ablated animals, the reversal frequency of ets-5 mutants did not significantly increase in response to a CO2 stimulus (Fig. 3A). The CO2-avoidance defect of ets-5 mutants was complemented by the ets-5::gfp transgene used to determine its cellular expression pattern (Fig. 3A). ets-5 is therefore necessary for acute CO2 avoidance, a behavior that is driven specifically by BAG neurons. The effect of CO2 on reversal frequencies of the different strains was calculated as an avoidance index, showing that BAG-ablated animals and ets-5 mutants were comparably insensitive to CO2 (Fig. 3B).

Figure 3

The BAG neurons of ets-5 mutants are defective in sensory transduction.

(A) ets-5 mutants are defective in a BAG-neuron-dependent CO2 avoidance behavior. Plotted are the mean fractions of animals ± SEM that reversed during a four second exposure to either control atmosphere (0% CO2, 20% O2, balance N2) or CO2-enriched atmosphere (10% CO2, 20% O2, balance N2). Strains tested were: the wild-type strain N2, the BAG-ablated strain CX11697, the ets-5 mutant strain FX1734, which carries the tm1734 deletion allele of ets-5, and a derivative of FX1734 that carries the ets-5::gfp transgene wzIs80. N = 3–5 populations of 30–50 animals. (B) The effect of ets-5 mutation on CO2 avoidance behavior is comparable to that of BAG neuron ablation. An avoidance index was calculated by subtracting the fraction of animals in a population that reversed in response to exposure to control atmosphere from the fraction that reversed in response to CO2-enriched atmosphere. Plotted are the mean avoidance indices for each of the four strains tested ± SEM. P values were calculated by one-way ANOVA. N = 3–5 populations of 30–50 animals. (C) Wild-type BAG neurons show robust calcium responses to a CO2 stimulus. Wild-type animals carrying a Prom transgene, which drives expression of cameleon specifically in BAG neurons, were immobilized and exposed to a 10 s pulse of 10% CO2. Plotted is the mean fractional ratio change in YFP/CFP emissions. The shaded area represents S.E.M. The cameleon expression transgene used was wzIs82. (D) The BAG neurons of ets-5 mutants show reduced calcium responses to a CO2 stimulus. Animals carrying a variant Prom transgene, which drives ets-5-independent expression of cameleon in BAG neurons, were immobilized and exposed to a 10 s pulse of 10% CO2. Plotted is the mean fractional ratio change in YFP/CFP emissions. The shaded area represents S.E.M. The cameleon expression transgene used was wzEx56.

To determine the effect of ets-5 mutation on the physiology of BAG neurons, we used the ratiometric calcium indicator cameleon YC3.60 [20] to monitor the responses of wild-type and mutant BAG neurons to CO2 stimuli. Wild-type BAG neurons showed robust responses to CO2 stimuli; the mean fractional change in the ratio of YFP to CFP emissions to a 10% CO2 stimulus was greater than 80% (Fig. 3C). By contrast, ets-5 mutant BAG neurons showed fractional ratio changes of less than 10% in response to the same CO2 stimulus (Fig. 3D). The BAG neurons of ets-5 mutants are, therefore, defective in sensory transduction at a point upstream of the generation of an intracellular calcium transient. We next tested whether the transduction defect of ets-5-mutant BAG neurons might be caused by defects in the regulation of components of the CO2-transduction pathway.

ETS-5 directly regulates the receptor-type guanylate cyclase gene gcy-9

The receptor-type cyclase GCY-9 is a critical component of the CO2 transduction pathway (17), and a Prom::gfp reporter transgene is not properly expressed by ets-5 mutants (Fig. 4A). Does ETS-5 directly regulate gcy-9? We first sought to identify sequences in the gcy-9 promoter that are required for expression by BAG neurons of a Prom::gfp reporter. We noted that the ETS domain of ETS-5 is highly similar to the ETS domain of the vertebrate transcription factor Pet1 (Fig. 4B), which functions in the specification of neurons in the vertebrate midbrain [21], [22], [23]. Pet1 preferentially binds to a consensus sequence ACCGGAAGTA [24], and the gcy-9 promoter contains a sequence that is almost identical to the reverse complement of this consensus sequence (Fig. 4C). Moreover, this putative ETS-binding site is highly conserved among nematode species, suggesting that it is functionally important (Figure 4C). This highly conserved ETS-site is adjacent to another putative ETS-binding site. We tested whether these presumptive ETS-binding sites are necessary for gcy-9 promoter function by introducing into the gcy-9 promoter a small deletion that removes them. We did not observe any activity of the mutant promoter in BAG neurons (Fig. 4D). We also introduced mutations in both ETS motifs, leaving the rest of the promoter intact. Like the deletion, these mutations abrogated expression of the reporter transgene, indicating that this motif is necessary for gcy-9 promoter function.

Figure 4

ETS-binding sites in the gcy-9 promoter are required for its BAG-neuron expression.

(A) Fluorescence micrographs of Prom expression in a wild-type animal and an ets-5 mutant. Prom expression was principally in BAG neurons (top panel) and was lost in the ets-5 mutant (bottom panel). In the lower panel, the BAGL neuron position is marked by a red circle. The scale bar in lower panel is 20 µm. A: anterior, V: ventral. The ets-5 mutant allele was tm1734. The Prom transgene was wzEx37. (B) The ETS domain of ETS-5 is most similar to that of mammalian Pet1. The ETS domains of ETS-5 and 26 mammalian ETS-family transcription factors were identified using the SMART database. Sequences were aligned using the CLUSTALW algorithm and sequence distances were plotted as a dendrogram using the Phylip sequence analysis package. (C) The gcy-9 promoter contains an evolutionarily conserved ETS-binding motif that is highly similar to the Pet1-binding consensus. A logo of the Pet1-binding consensus sequence [24] is shown aligned to sequences approximately 200 bp upstream of gcy-9 coding sequences from C. elegans and three other rhabditid nematodes. (D) ETS binding motifs in the gcy-9 promoter are required for promoter function. Shown are ventral views of adult hermaphrodites carrying a 1.9 kb fragment of the gcy-9 promoter fused to gfp coding sequences, or a promoter variant that either carries an 88 bp deletion centered around the ETS binding site at −202 bp, or a variant that carries four-base substitutions in the ETS-binding sites. In each case two lines were tested for GFP expression. Red circles indicate the location of BAG neurons in the animal carrying the mutant transgene. The wild-type gcy-9 promoter transgene used was wzEx37 and the mutant promoter transgene was wzEx38.

We next tested whether ETS-5 associates in vivo with the gcy-9 promoter at the ETS sites using chromatin immunoprecipitation. ETS-5::GFP immunoprecipitates were enriched for sequences containing the ETS binding site from the gcy-9 promoter in comparison to precipitates prepared from extracts of non-transgenic animals, which did not express ETS-5::GFP (Fig. 5A). By contrast, sequences upstream of the ETS site were not significantly enriched in ETS-5::GFP immunoprecipitates. ETS-5, therefore, associates with a highly conserved and functionally important sequence element in the gcy-9 promoter. To determine whether ETS-5 directly interacts with this element, we performed electrophoretic mobility shift assays (EMSAs) using a synthetic 45 base-pair DNA duplex containing the ETS binding site and recombinant ETS-5 protein (Fig. 5B). Recombinant ETS-5 altered the mobility of the duplex, and incorporation of labeled duplex into the complex was efficiently blocked by an excess of unlabeled duplex but not by sequence-scrambled duplex (Fig. 5B). Taken together with the observation that these ETS-binding motifs are required for the function of the gcy-9 promoter (Fig. 4D), these data indicate that a direct interaction between ETS-5 and sequences in the gcy-9 promoter is required for expression of gcy-9 in BAG neurons.

Figure 5

ETS-5 directly interacts with the gcy-9 promoter.

(A) ETS-5::GFP associates with the gcy-9 promoter in vivo. Anti-GFP immunoprecipitates were prepared from cross-linked extracts of wild-type animals or animals carrying a functional ets-5::gfp transgene and interrogated for the presence of gcy-9 promoter sequences by PCR. Immunoprecipitates from transgenic animals were enriched for gcy-9 promoter sequences that contained the ETS-binding site at −202 bp. Control sequences at −5000 bp were not enriched in immunoprecipitates from transgenic animals. The ets-5::gfp transgene used was wzIs80. (B) ETS-5 binds to gcy-9 promoter sequences in vitro. A mobility shift assay was performed with recombinant GST::ETS-5 and a 45 bp biotinylated DNA duplex probe containing the ETS-binding site from the gcy-9 promoter. Recombinant GST::ETS-5 but not GST alone altered the electrophoretic mobility of the probe. The interaction between GST::ETS-5 and the probe was blocked by a molar excess of unlabeled probe but not by an excess of scrambled probe with the same nucleotide composition. Excess unlabeled wild-type and scrambled competitor probe was added in the following molar ratios: 10×, 50×, 100×, 500×.

Expression of gcy-9 bypasses a requirement for ets-5 in CO2-avoidance

Are ets-5 mutants defective in CO2 sensing principally because they fail to express gcy-9, or is gcy-9 one of many BAG-cell-specific genes that are regulated by ETS-5 and necessary for CO2 chemosensation? To answer this question, we used promoters that are not regulated by ETS-5 to express gcy-9 in neurons that (1) mediate an acute avoidance behavior similar to that triggered by BAG neurons and (2) like BAG neurons, use cGMP signaling. The gcy-36 promoter is active in the URX oxygen-sensing neurons [25], which drive reversals in response to a hyperoxic stimulus [11]. The gcy-18 promoter is specifically active in thermosensory AFD neurons [26], which also drive reversals [27]. Activity of neither the gcy-18 nor the gcy-36 promoter was affected by ets-5 mutation (Fig. 6A). We then tested whether these promoters driving expression of gcy-9 could rescue the CO2 avoidance defect of ets-5 mutants. Unlike non-transgenic ets-5 mutants, which failed to increase reversal frequency in response to a CO2 stimulus, ets-5 mutants carrying either a Prom transgene or a Prom transgene showed robust avoidance responses to CO2 (Fig. 6B). A requirement for ets-5 in CO2-avoidance behavior can therefore be bypassed by expressing the ETS-5 target gene gcy-9 in neurons that are specified by ets-5-independent mechanisms.

Figure 6

Expression of the ETS-5 target gene gcy-9 restores CO2-chemosensitivity to ets-5 mutants.

(A) gcy-36 and gcy-18 promoters, which are active in oxygen-sensing and thermosensory neurons, respectively, are not regulated by ETS-5. Shown are lateral views of wild-type and ets-5 mutant animals carrying either a gcy-36 reporter, which is expressed by the oxygen-sensing URX neurons, or a gcy-18 reporter, which is expressed by the thermosensory AFD neurons. A: anterior, V: ventral, scale bar is 20 µm. The Prom transgene used was wzEx39 and the Prom transgene was wzEx40. (B) Expression of gcy-9 in either the URX oxygen sensors or the AFD thermosensors rescues the behavioral defect of ets-5 mutants. The left plot shows the mean fraction of animals ± SEM that reversed during a four second exposure to either control atmosphere (0% CO2, 20% O2, balance N2) or CO2-enriched atmosphere (10% CO2, 20% O2, balance N2). The right plot shows the effect of CO2 on reversals as measured with an avoidance index, as in Fig. 2C. Plotted are the mean avoidance indices for each of the four strains tested ± SEM. P values were calculated by one-way ANOVA. N = 3–5 populations of 30–50 animals. The ets-5 mutant strain used was FX1734. The Prom gcy ::gcy-9 transgene used was wzIs97 and the Prom transgene was wzEx34. (C) Expression of gcy-9 in the URX oxygen sensors confers sensitivity to CO2. The ratiometric calcium indicator cameleon was expressed in the URX neurons. Wild-type URX neurons (top panel) showed a small decrease in the ratio of YFP:CFP emissions in response to 10 s CO2 pulses indicating decreases in cell calcium. URX neurons expressing gcy-9 showed increases in cell calcium in response to CO2 stimuli, with an average ratio change of greater than 20% (lower panel). The cameleon expression transgene used was wzIs96[Prom and the gcy-9 expression transgene was wzIs97[Prom (D) Expression of gcy-9 in the AFD thermosensors confers sensitivity to CO2. Calcium responses of wild-type AFD neurons (top panel) and AFD neurons that express gcy-9 (bottom panel) in responses to a 10% CO2 stimulus. The cameleon expression transgene was fxIs105[Prom, and the Prom transgene was wzEx34. For panels C and D, plots are mean YFP/CFP emissions ratios normalized to the pre-stimulus ratio R0 (N = 16–22 animals). Red shaded areas represent SEM.

Are URX and AFD neurons expressing the ETS-5 target gene gcy-9 now CO2 chemosensors? We monitored the activity of wild-type and gcy-9-expressing URX and AFD neurons using in vivo calcium imaging. Wild-type URX neurons showed small decreases in cell calcium in response to a CO2 stimulus. By contrast, URX neurons of transgenic animals showed calcium increases that were phase-locked to the CO2 stimulus (Fig. 6C). We performed the same measurements on wild-type and transgenic AFD thermosensory neurons (Fig. 6D). Under some experimental conditions, AFD neurons demonstrate a calcium response to the falling edge of a CO2 pulse [14] i.e. an ‘off’ response. Under our experimental conditions, wild-type AFD neurons showed little response to CO2 stimuli (Fig. 6D). Transgenic AFD neurons expressing gcy-9, however, showed robust calcium responses to CO2 stimuli (Fig. 4D), demonstrating that expression of gcy-9 in AFDs rendered them sensitive to CO2. Expression of the ETS-5 target gene, gcy-9 is therefore sufficient to confer CO2 chemosensitivity upon neurons that do not normally respond to CO2.

Discussion

Together, our data suggest that the mechanism by which ETS-5 determines CO2-sensitivity is to drive the expression of the receptor-type guanylate cyclase GCY-9, which likely functions as a receptor either for CO2 or for a CO2 metabolite such as bicarbonate or free protons. We previously showed that BAG neuron activation by CO2 requires cyclic-nucleotide-gated ion channels [17], suggesting that CO2-sensing by BAG neurons minimally requires a two-component transduction pathway: a CO2-activated guanylate cyclase and a cGMP-gated ion channel. Could such a transduction pathway exist in other species? Mammalian genomes encode receptor-type cyclases related to GCY-9. These cyclases have been matched to diverse ligands [28] and are also known to be regulated by interactions with anions, including chloride [29] and bicarbonate [30], [31], [32], [33]. Because vertebrate cyclases are modulated by the CO2 metabolite bicarbonate, this mechanism of cyclase activation might lie at the heart of an evolutionarily conserved pathway for CO2 detection.

Our data show that gcy-9 expression is a determinant of whether a C. elegans neuron is CO2-chemosensitive. How is expression of gcy-9 restricted to the BAG chemosensors? ETS-5 is necessary for expression of gcy-9 but is also expressed by neurons that do not express gcy-9 (Fig. 2). ETS-5 must therefore cooperate with additional, cell-type-specific factors to permit the activation of the gcy-9 promoter specifically in BAG neurons. For example, overlapping expression of ETS-5 and a co-regulatory partner only in BAG neurons might drive cell-type-specific expression of GCY-9. Similar context-dependent neuronal specification events are known to require such ‘combinatorial coding’ [34]. Indeed, in other contexts, ETS-family transcription factors interact with co-regulatory transcription factors, including LIM-domain, homeodomain and JUN proteins [35], [36], [37]. Further studies will identify ETS-5 interacting partners that specifically drive expression of gcy-9 in BAG neurons and consequently determine their CO2-chemosensitivity.

ETS-5 has the same domain structure as the vertebrate ETS protein Pet1, and its ETS domain, which mediates sequence-specific interactions with DNA [38], is almost identical to that of Pet1. Like ETS-5, Pet1 functions in the specification of neuronal cell fates [23]. Notably, among the neurons that are specified by Pet1 are serotonergic neurons, that are directly activated by CO2 and have recently been shown to function in vivo in the respiratory CO2 chemoreflex [21], [22]. In addition to promoting a serotonergic identity, Pet1, like ETS-5, might regulate the expression of genes that constitute a molecular pathway dedicated to the detection of CO2.

Materials and Methods

Strains used in this study

Strains used in this study are listed in Table S1. Strains were grown on NGM agar at 25°C on Escherichia coli OP50 except when used for chromatin immunoprecipitation experiments (see below). Transgenic animals were created according to Mello et al. [39]. Prom coinjection marker was used at 5 ng ul−1. Prom was used at 25 ng ul−1. Prom was used at 30 ng ul−1. The lin-15 rescuing plasmid pL15EK was used at 30 ng ul−1. Prom was used at 3 ng ul−1. rol-6 was used at 100 ng ul−1. GFP, cameleon, dsRed and GCY-9 expression plasmids were injected at 100 ng ul−1. In some cases, extrachromosomal transgenes were integrated using gamma radiation (5000 rads).

Plasmids

A complete list of plasmids used in this study is in Table S2.

Microscopy

Young adults were anaesthetized with 30 mM sodium azide and mounted on a 2% agarose pad made in M9 medium. Series of confocal images were obtained with a Zeiss LSM510 microscope and maximum-projection images were created using ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2011).

Behavioral Assay for Acute CO2 Avoidance

Behavioral assays were carried out essentially as described by Hallem and Sternberg [15]. Three or four trials of 30–50 animals were performed for each genotype. Animals were picked onto unseeded 6 cm nematode growth medium (NGM) agar plates. Gases were certified mixes of 20% oxygen with or without 10% CO2, balance nitrogen (Airgas).

Statistical analysis

Standard errors and P values were calculated using GraphPad Prism analysis software. P values were calculated from a one-way ANOVA analysis of avoidance indexes.

Chromatin immunoprecipitation

FQ237 wzIs80[ets-5::gfp lin-15AB(+)]; lin-15AB(n765) and N2 animals were grown on 10 cm peptone plates seeded with OP50. A mixed-stage culture of animals was repeatedly washed in M9 and harvested by centrifugation. 5 ml pellets of worms were resuspended in an equal volume of PBS+protease inhibitors and flash-frozen in liquid nitrogen. Frozen pellets were stored at −80°C prior to lysis.

To prepare extracts, frozen pellets were pulverized in a CryoCup (BioSpec) and cross-linked for 10 minutes by addition of 1.1% formaldehyde in PBS+PMSF. Formaldehyde was quenched by addition of 2.5 M glycine in PBS for 5 minutes. Cross-linked extracts were then washed in PBS, resuspended in FA buffer+0.1% sarkosyl, and sonicated using a Branson sonifier set to 35% output, 90% duty cycle with 8 sets of 12 pulses. Cross-linked sonicated extracts were then frozen at −80°C.

Prior to immunoprecipitation, extracts were thawed on ice. 5 µg of mouse anti-GFP IgG (Roche) was added to cross-linked sonicated extracts, incubated overnight at 4°C, and recovered using protein G agarose beads (Roche). Beads were washed as described by Ercan et al. [40] and immune complexes were eluted in 150 µl of 100 mM glycine pH 2.5. Protein-DNA crosslinks were reversed by incubation with proteinase K (Qiagen) at 65°C overnight. Nucleic acids were recovered using Qiaquick columns and eluted in 40 µl of elution buffer.

gcy-9 promoter sequences were detected in immunoprecipitates using PCR. To detect the ETS site at −202 base pairs we used the primers 5′-GTTGCATTAGACAGTGTCATAG-3′ and 5′-CCGCAAACAGTAACAATTC-3′. To detect control sequences we used primers 5′-CAATGAGCTGTCCTGGCTTC- 3′ and 5′-TCGACATCTTTGACGCTGCTC-3′. 5 µl of sample from the chromatin immunoprecipitation was used as a template for a 30-cycle PCR.

Electrophoretic mobility shift assay

Recombinant GST::ETS-5 was expressed in E. coli strain BL21and purified using glutathione sepharose affinity chromatography (GE Healthcare). 1 µg of recombinant ETS-5 or 2 µg of GST was added to each reaction, which contained 0.1 pmol of 3′-biotin-labeled duplex probe. In some reactions, a molar excess of unlabeled competitor duplex (identical or sequence-scrambled) was included. Reactions were incubated at room temperature for 20 minutes and protein-DNA complexes were separated from unbound probe by electrophoresis in a 6% polyacrylamide gel in 0.5× TBE. Nucleic acids were then transferred to a nylon membrane (Amersham, GE Healthcare) and detected using HRP-coupled streptavidin and enhanced chemiluminscence (Pierce Protein Research Products).

The sequence of the biotinylated probe and the unlabeled competitor was:

5′-ATCCACTTCCGATGGGCCCTTCCGGCATGATAAGAAGTGAGTGGC-3′.

The sequence of the scrambled competitor was:

5′-CTGACCCCGTGTCGCTAATAGACTCAGTTACGCAAGATGG-3′.

in vivo calcium imaging

Calcium imaging was performed essentially as previously described [17]. Young adults were immobilized with cyanoacrylate veterinary glue (Surgi-Lock; Meridian Animal Health) on a cover glass coated with a 2% agarose pad made with 10 mM HEPES (pH 7.4). The cover glass was affixed to a custom-made air chamber. The specimen was illuminated with 435-nm excitation light and imaged using a 40× Nikon long-working distance objective (0.75 numerical aperture). The emission image was passed through a DV2 image splitter (Photometrics), and the CFP and YFP emission images were projected onto two halves of a cooled CCD camera (Andor). Images were acquired at 10 Hz, with exposure times between 10 and 50 ms. Gas perfusion was controlled by three-way valves (Numatics) driven by a custom-made valve controller unit. Excitation light, image acquisition, and hardware control were performed by the Live Acquisition software package (Till Photonics). Post-acquisition analysis of ratio plots was performed using custom Matlab scripts, which subtracted linear baseline drift from traces and applied a five-frame boxcar filter to the ratio time series. Custom certified gas mixes used for imaging were obtained from Airgas.

Deletion analysis of the promoter. (A) Genomic locus of the flp-17 gene with the location of predicted ETS binding sites shown as blue bars. mCherry protein was driven by the promoter elements indicated with black bars and expression in the BAG neurons was scored. The Prom9 is a 138 bp fragment that is sufficient to drive expression of mCherry protein in the BAG neurons. N≥30 animals/transgenic line. (B) Dorsal view of Prom9 expression in a wild-type animal. BAG neuron positions are marked by red circles. The scale bar is 20 µm.

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mutants have defects in expression of multiple BAG-neuron genes. (A) An independently derived allele of ets-5 has BAG neurons that fail to express a Prom transgene. The fraction of transgenic ets-5(tm866) and ets-5(tm1734) animals that express GFP in BAG neurons is plotted next to the wild type (+) and ets-5(tm1734) mutants carrying rescuing transgenes derived from a fosmid that encompasses the ets-5 locus. N≥30, # = independent transgenic lines. (B) Dorsal views of Prom expression in a wild-type animal and an ets-5 mutant. Prom expression was lost in the ets-5 mutant (bottom panel). BAG neuron positions are marked by red circles. The scale bar in lower panel is 20 µm. A: anterior, V: ventral. The ets-5 mutant allele was tm1734. The Prom transgene was ynIs37. (C) Lateral views of Prom expression in a wild-type animal and an ets-5 mutant. Prom expression was lost in the ets-5 mutant (bottom panel). BAG neuron position in the lower panel is marked by a red circle. The scale bar in lower panel is 20 µm. A: anterior, V: ventral. The ets-5 mutant allele was tm1734. The Prom transgene was ynIs64. (D) Lateral views of Prom expression in a wild-type animal and an ets-5 mutant. Prom expression was lost in the ets-5 mutant (bottom panel). BAG neuron position in the lower panel is marked by a red circle. The scale bar in lower panel is 20 µm. A: anterior, V: ventral. The ets-5 mutant allele was tm1734. The Prom transgene was ynIs64.

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Characterization of the transcript. (A) Genomic organization of ets-5 coding sequences as determined by amplification of ets-5 cDNA using gene-specific and SL1 primers. cDNAs derived from SL1 trans-spliced messages contained 95 bases of 5′ non-coding sequence (white box) and were organized into five exons. Brackets indicate regions deleted by the tm1734 and tm866 mutations. (B) Nucleotide sequence of ets-5 cDNA. The SL1 leader sequence is underlined. Non-coding sequences are lowercase and exons are denoted by text boxes alternately colored blue and green. (C) Predicted amino acid sequence of ETS-5. Sequences of the ETS homology domain are in white text on a black background.

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Strains used in this study. Strain designations and complete genotypes of all the strains used in this study.

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Plasmids used in this study. Complete descriptions of plasmids used in this studies and the sequences of primers used for their construction.

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