Manisha Rajan Madkaikar1, Maya Gupta, Meghana Rao, Kanjaksha Ghosh. 1. National Institute of Immunohematlogy, Pediatric Immunology and Leukocyte Biology, 13th Flr, KEM Hospital, Parel, Mumbai, Maharashtra, 400012, India. madkaikarm@icmr.org.in
Abstract
OBJECTIVES: To optimize a simple flowcytometric technique for Prenatal diagnosis (PND) for Leukocyte adhesions defect (LAD-I) on cordocentesis sample at 18 wk gestation. METHODS: Normal reference ranges for expression of CD18/CD11-integrins in neutrophils and lymphocytes at 18 wk of gestation were established by flowcytometry. PND for LAD-I was then performed on the cordocentesis samples in three 'at risk' pregnancies after ruling out maternal contamination. RESULTS: CD18 and CD11a expression on fetal lymphocytes were found to be the most useful parameters for PND of LAD-I. All the three fetuses tested showed normal expression of CD18/CD11-integrins and thus were unaffected. This was confirmed by testing the cord blood (CB) samples after delivery and normal growth and absence of serious infections on follow-up. CONCLUSIONS: Flowcytometry offers a rapid and sensitive technique for PND of LAD-I in the absence of facilities for molecular diagnosis. Obstetricians, even in developing countries with modest facilities, can offer considerable relief for the families.
OBJECTIVES: To optimize a simple flowcytometric technique for Prenatal diagnosis (PND) for Leukocyte adhesions defect (LAD-I) on cordocentesis sample at 18 wk gestation. METHODS: Normal reference ranges for expression of CD18/CD11-integrins in neutrophils and lymphocytes at 18 wk of gestation were established by flowcytometry. PND for LAD-I was then performed on the cordocentesis samples in three 'at risk' pregnancies after ruling out maternal contamination. RESULTS:CD18 and CD11a expression on fetal lymphocytes were found to be the most useful parameters for PND of LAD-I. All the three fetuses tested showed normal expression of CD18/CD11-integrins and thus were unaffected. This was confirmed by testing the cord blood (CB) samples after delivery and normal growth and absence of serious infections on follow-up. CONCLUSIONS: Flowcytometry offers a rapid and sensitive technique for PND of LAD-I in the absence of facilities for molecular diagnosis. Obstetricians, even in developing countries with modest facilities, can offer considerable relief for the families.
Authors: D C Anderson; F C Schmalsteig; M J Finegold; B J Hughes; R Rothlein; L J Miller; S Kohl; M F Tosi; R L Jacobs; T C Waldrop Journal: J Infect Dis Date: 1985-10 Impact factor: 5.226
Authors: D C Anderson; F C Schmalstieg; M A Arnaout; S Kohl; M F Tosi; N Dana; G J Buffone; B J Hughes; B R Brinkley; W D Dickey Journal: J Clin Invest Date: 1984-08 Impact factor: 14.808