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Literature DB >> 22475983

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate.

Keiichi Ohtsuka¹, Shinobu Sato, Yusuke Sato, Kojiro Sota, Shinsuke Ohzawa, Tomoki Matsuda, Kiwamu Takemoto, Nobutoki Takamune, Bernard Juskowiak, Takeharu Nagai, Shigeori Takenaka.

Abstract

When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell. This journal is © The Royal Society of Chemistry 2012

Entities: Chemical Gene

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Year: 2012 PMID： 22475983 DOI： 10.1039/c2cc30536d

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

11 in total

Review 1. Aptamer-based molecular imaging.

Authors: Tianjiao Wang; Judhajeet Ray
Journal: Protein Cell Date: 2012-09-15 Impact factor: 14.870

2. G-ruption: the third international meeting on G-quadruplex and G-assembly.

Authors: Liliya A Yatsunyk; Tracy M Bryan; F Brad Johnson
Journal: Biochimie Date: 2012-09-10 Impact factor: 4.079

Review 3. Applications of aptamers in targeted imaging: state of the art.

Authors: Casey A Dougherty; Weibo Cai; Hao Hong
Journal: Curr Top Med Chem Date: 2015 Impact factor: 3.295

Review 4. Nucleic-Acid Structures as Intracellular Probes for Live Cells.

Authors: Devleena Samanta; Sasha B Ebrahimi; Chad A Mirkin
Journal: Adv Mater Date: 2019-07-04 Impact factor: 30.849

5. Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes.

Authors: Arun A Tanpure; Seergazhi G Srivatsan
Journal: Nucleic Acids Res Date: 2015-07-21 Impact factor: 16.971

10. The exception that confirms the rule: a higher-order telomeric G-quadruplex structure more stable in sodium than in potassium.

Authors: Carole Saintomé; Samir Amrane; Jean-Louis Mergny; Patrizia Alberti
Journal: Nucleic Acids Res Date: 2016-01-13 Impact factor: 16.971

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate.

Review 1. Aptamer-based molecular imaging.

2. G-ruption: the third international meeting on G-quadruplex and G-assembly.

Review 3. Applications of aptamers in targeted imaging: state of the art.

Review 4. Nucleic-Acid Structures as Intracellular Probes for Live Cells.

5. Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes.

6. Rational design of a structure-switching DNA aptamer for potassium ions.

7. Fluorescence-based tools to probe G-quadruplexes in cell-free and cellular environments.

8. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration.

9. Network topology-directed design of molecular CPU for cell-like dynamic information processing.

10. The exception that confirms the rule: a higher-order telomeric G-quadruplex structure more stable in sodium than in potassium.