AIM: To investigate the relationship of alveolar macrophages and inhaled nanoparticles (NPs) in the lung. MATERIALS & METHODS: Rats were exposed by inhalation to 16-nm gold NPs for 6 h, and ultramicroscopic observation on the frequency and localization of gold NPs within lavaged macrophages was performed for 7 days. RESULTS & DISCUSSION: The majority of macrophages examined on day 0 (94%) contained internalized gold NPs, and the percentage decreased to 59% on day 7. Gold NPs were exclusively found within cytoplasmic vesicles. On day 0, most gold NPs appeared to be individual or slightly agglomerated, while they were frequently agglomerated on day 7. CONCLUSION: Alveolar macrophages efficiently internalized NPs by endocytosis, and rearrangements of vesicles and of NPs in the vesicles of macrophages occurred.
AIM: To investigate the relationship of alveolar macrophages and inhaled nanoparticles (NPs) in the lung. MATERIALS & METHODS:Rats were exposed by inhalation to 16-nm gold NPs for 6 h, and ultramicroscopic observation on the frequency and localization of gold NPs within lavaged macrophages was performed for 7 days. RESULTS & DISCUSSION: The majority of macrophages examined on day 0 (94%) contained internalized gold NPs, and the percentage decreased to 59% on day 7. Gold NPs were exclusively found within cytoplasmic vesicles. On day 0, most gold NPs appeared to be individual or slightly agglomerated, while they were frequently agglomerated on day 7. CONCLUSION: Alveolar macrophages efficiently internalized NPs by endocytosis, and rearrangements of vesicles and of NPs in the vesicles of macrophages occurred.
Authors: Marianne Geiser; Christoph Wigge; Melanie L Conrad; Sylvie Eigeldinger-Berthou; Lisa Künzi; Holger Garn; Harald Renz; Marcus A Mall Journal: BMC Pulm Med Date: 2014-07-16 Impact factor: 3.317
Authors: Marianne Geiser; Oliver Quaile; Alexander Wenk; Christoph Wigge; Sylvie Eigeldinger-Berthou; Stephanie Hirn; Martin Schäffler; Carsten Schleh; Winfried Möller; Marcus A Mall; Wolfgang G Kreyling Journal: Part Fibre Toxicol Date: 2013-05-16 Impact factor: 9.400
Authors: Regine Gradl; Martin Dierolf; Benedikt Günther; Lorenz Hehn; Winfried Möller; David Kutschke; Lin Yang; Martin Donnelley; Rhiannon Murrie; Alexander Erl; Tobias Stoeger; Bernhard Gleich; Klaus Achterhold; Otmar Schmid; Franz Pfeiffer; Kaye Susannah Morgan Journal: Sci Rep Date: 2018-05-01 Impact factor: 4.379