Literature DB >> 22470153

Two-photon imaging of the immune system.

Ivan L Dzhagalov1, Heather J Melichar, Jenny O Ross, Paul Herzmark, Ellen A Robey.   

Abstract

Two-photon microscopy is a powerful method for visualizing biological processes as they occur in their native environment in real time. The immune system uniquely benefits from this technology as most of its constituent cells are highly motile and interact extensively with each other and with the environment. Two-photon microscopy has provided many novel insights into the dynamics of the development and function of the immune system that could not have been deduced by other methods and has become an indispensible tool in the arsenal of immunologists. In this unit, we provide several protocols for preparation of various organs for imaging by two-photon microscopy that are intended to introduce the new user to some basic aspects of this method.

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Year:  2012        PMID： 22470153      PMCID： PMC3662370          DOI： 10.1002/0471142956.cy1226s60

Source DB:  PubMed          Journal:  Curr Protoc Cytom        ISSN： 1934-9297


  25 in total

1.  Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.

Authors:  B C Schaefer; M L Schaefer; J W Kappler; P Marrack; R M Kedl
Journal:  Cell Immunol       Date:  2001-12-15       Impact factor: 4.868

2.  Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.

Authors:  Philippe Bousso; Nirav R Bhakta; Richard S Lewis; Ellen Robey
Journal:  Science       Date:  2002-06-07       Impact factor: 47.728

3.  Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes.

Authors:  Philippe Bousso; Ellen Robey
Journal:  Nat Immunol       Date:  2003-05-05       Impact factor: 25.606

4.  Multiphoton intravital microscopy to study lymphocyte motility in lymph nodes.

Authors:  Thomas T Murooka; Thorsten R Mempel
Journal:  Methods Mol Biol       Date:  2012

5.  Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages.

Authors:  N Faust; F Varas; L M Kelly; S Heck; T Graf
Journal:  Blood       Date:  2000-07-15       Impact factor: 22.113

6.  Thymic origin of intestinal alphabeta T cells revealed by fate mapping of RORgammat+ cells.

Authors:  Gérard Eberl; Dan R Littman
Journal:  Science       Date:  2004-07-09       Impact factor: 47.728

7.  Visualizing dendritic cell networks in vivo.

Authors:  Randall L Lindquist; Guy Shakhar; Diana Dudziak; Hedda Wardemann; Thomas Eisenreich; Michael L Dustin; Michel C Nussenzweig
Journal:  Nat Immunol       Date:  2004-11-14       Impact factor: 25.606

8.  Directed migration of positively selected thymocytes visualized in real time.

Authors:  Colleen M Witt; Subhadip Raychaudhuri; Brian Schaefer; Arup K Chakraborty; Ellen A Robey
Journal:  PLoS Biol       Date:  2005-05-03       Impact factor: 8.029

9.  Spontaneous skin ulceration and defective T cell function in CD18 null mice.

Authors:  K Scharffetter-Kochanek; H Lu; K Norman; N van Nood; F Munoz; S Grabbe; M McArthur; I Lorenzo; S Kaplan; K Ley; C W Smith; C A Montgomery; S Rich; A L Beaudet
Journal:  J Exp Med       Date:  1998-07-06       Impact factor: 14.307

10.  Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal.

Authors:  Anna-Katerina Hadjantonakis; Suzanne Macmaster; Andras Nagy
Journal:  BMC Biotechnol       Date:  2002-06-11       Impact factor: 2.563
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  25 in total

1.  Distinct temporal patterns of T cell receptor signaling during positive versus negative selection in situ.

Authors:  Heather J Melichar; Jenny O Ross; Paul Herzmark; Kristin A Hogquist; Ellen A Robey
Journal:  Sci Signal       Date:  2013-10-15       Impact factor: 8.192

2.  Nanobody-Antigen Conjugates Elicit HPV-Specific Antitumor Immune Responses.

Authors:  Andrew W Woodham; Ross W Cheloha; Jingjing Ling; Mohammad Rashidian; Stephen C Kolifrath; Maia Mesyngier; Joao N Duarte; Justin M Bader; Joseph G Skeate; Diane M Da Silva; W Martin Kast; Hidde L Ploegh
Journal:  Cancer Immunol Res       Date:  2018-05-23       Impact factor: 11.151

3.  Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread.

Authors:  Janine L Coombes; Brittany A Charsar; Seong-Ji Han; Joanna Halkias; Shiao Wei Chan; Anita A Koshy; Boris Striepen; Ellen A Robey
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-06       Impact factor: 11.205

4.  T cell progenitor therapy-facilitated thymopoiesis depends upon thymic input and continued thymic microenvironment interaction.

Authors:  Michelle J Smith; Dawn K Reichenbach; Sarah L Parker; Megan J Riddle; Jason Mitchell; Kevin C Osum; Mahmood Mohtashami; Heather E Stefanski; Brian T Fife; Avinash Bhandoola; Kristin A Hogquist; Georg A Holländer; Juan Carlos Zúñiga-Pflücker; Jakub Tolar; Bruce R Blazar
Journal:  JCI Insight       Date:  2017-05-18

5.  Distinct phases in the positive selection of CD8+ T cells distinguished by intrathymic migration and T-cell receptor signaling patterns.

Authors:  Jenny O Ross; Heather J Melichar; Byron B Au-Yeung; Paul Herzmark; Arthur Weiss; Ellen A Robey
Journal:  Proc Natl Acad Sci U S A       Date:  2014-06-09       Impact factor: 11.205

Review 6.  Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior.

Authors:  Miles A Miller; Ralph Weissleder
Journal:  Adv Drug Deliv Rev       Date:  2016-06-04       Impact factor: 15.470

7.  Two-photon imaging of cellular dynamics in the mouse spinal cord.

Authors:  Jason G Weinger; Milton L Greenberg; Melanie P Matheu; Ian Parker; Craig M Walsh; Thomas E Lane; Michael D Cahalan
Journal:  J Vis Exp       Date:  2015-02-22       Impact factor: 1.355

8.  Stable interactions and sustained TCR signaling characterize thymocyte-thymocyte interactions that support negative selection.

Authors:  Heather J Melichar; Jenny O Ross; Kayleigh T Taylor; Ellen A Robey
Journal:  J Immunol       Date:  2014-12-17       Impact factor: 5.422

9.  Opposing chemokine gradients control human thymocyte migration in situ.

Authors:  Joanna Halkias; Heather J Melichar; Kayleigh T Taylor; Jenny O Ross; Bonnie Yen; Samantha B Cooper; Astar Winoto; Ellen A Robey
Journal:  J Clin Invest       Date:  2013-04-15       Impact factor: 14.808

Review 10.  Tracing Antiviral CD8+ T Cell Responses Using In Vivo Imaging.

Authors:  Courtney S Malo; Heather D Hickman
Journal:  J Immunol       Date:  2019-08-15       Impact factor: 5.422
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