OBJECTIVE: In Indian HIV-infected patients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. METHODS: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. RESULTS: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. CONCLUSIONS: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infected patients with lower dependence on mitogen-response and CD4 counts.
OBJECTIVE: In Indian HIV-infectedpatients, IP-10 response to QuantiFERON-TB Gold In tube (QFT-IT) antigens has been associated to tuberculosis (TB). However, specificity for active TB was lower than that reported by QFT-IT, making accuracy for TB detection questionable. To investigate this uncertainty, likely due to India being highly endemic for TB, and to better identify TB correlates, we evaluated the IP-10-based assay in HIV-infected subjects in Italy, a low-TB endemic country. METHODS: 195 individuals were prospectively enrolled; 118 were HIV-infected (21 with active TB, 97 without active TB, and distinguished as high/low-TB-risk). QFT-IT was performed and IP-10 was evaluated by ELISA. RESULTS: Among the HIV-infected individuals, sensitivity for active TB was 66.7% by IP-10-based test and 52.4% (p = 1) by QFT-IT. IP-10-based assay showed a lower dependence on mitogen-response and CD4 counts than QFT-IT. Among subjects without active TB, a higher proportion of IP-10 responders was shown in high-TB-risk subjects than low-TB-risk subjects (40.0% vs 12.9%), similar to QFT-IT (37.1% vs 4.8%). Low-TB risk subjects showed 87.1% specificity for active TB by IP-10-based test vs 95.2% by QFT-IT. CONCLUSIONS: In a low-TB endemic country, besides IFN-γ, IP-10 response to QFT-IT is associated with active TB and TB risk factors in HIV-infectedpatients with lower dependence on mitogen-response and CD4 counts.
Authors: Camilla H Drabe; Lasse S Vestergaard; Marie Helleberg; Nyagonde Nyagonde; Michala V Rose; Filbert Francis; Ola P Theilgaard; Jens Asbjørn; Ben Amos; Ib Christian Bygbjerg; Morten Ruhwald; Pernille Ravn Journal: Am J Trop Med Hyg Date: 2016-02-01 Impact factor: 2.345
Authors: Linda Petrone; Angela Cannas; Francesco Aloi; Martin Nsubuga; Joseph Sserumkuma; Ritah Angella Nazziwa; Levan Jugheli; Tedson Lukindo; Enrico Girardi; Klaus Reither; Delia Goletti Journal: Biomed Res Int Date: 2015-08-05 Impact factor: 3.411
Authors: Clíona Ní Cheallaigh; Ian Fitzgerald; Jacinta Grace; Gurmit Jagjit Singh; Nahla El-Eraki; Noel Gibbons; Joseph Keane; Thomas R Rogers; Susan Clarke; Colm Bergin Journal: PLoS One Date: 2013-01-30 Impact factor: 3.240
Authors: Irene Andia Biraro; Simon Kimuda; Moses Egesa; Stephen Cose; Emily L Webb; Moses Joloba; Steven G Smith; Alison M Elliott; Hazel M Dockrell; Achilles Katamba Journal: PLoS One Date: 2016-01-15 Impact factor: 3.240