ETHNOPHARMACOLOGICAL RELEVANCE: The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago major L. (plantain) on cell proliferation and migration in vitro, as a test for potential wound healing properties. MATERIALS AND METHODS: Water and ethanol-based extracts were prepared from Plantago major fresh and dried leaves, and tested in vitro in a scratch assay with oral epithelial cells. RESULTS: The scratch assay produced reliable results after 18 h. Most of the tested extracts increased the proliferation/migration of the oral epithelial cells compared to the negative control. A concentration of 1.0 mg/mL (on dry weight basis) appears to be optimal regardless of type of extract, and among the alternatives, 0.1 mg/mL was always better than 10 mg/mL. Ethanol-based extracts with a concentration of 10 mg/mL had very detrimental effects on cell proliferation/migration. At the other two concentrations, ethanol-based extracts had the most beneficial effect, followed by water extracts of fresh leaves, ethanol plus water extracts of dried leaves and, finally, water extracts of dried leaves. CONCLUSIONS: This study suggests that both the water extracts and the more polyphenol-rich ethanol-based extracts of Plantago major leaves have medicinal properties. Further research is, however, needed to determine what compounds are responsible for the wound healing effects.
ETHNOPHARMACOLOGICAL RELEVANCE: The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago major L. (plantain) on cell proliferation and migration in vitro, as a test for potential wound healing properties. MATERIALS AND METHODS:Water and ethanol-based extracts were prepared from Plantago major fresh and dried leaves, and tested in vitro in a scratch assay with oral epithelial cells. RESULTS: The scratch assay produced reliable results after 18 h. Most of the tested extracts increased the proliferation/migration of the oral epithelial cells compared to the negative control. A concentration of 1.0 mg/mL (on dry weight basis) appears to be optimal regardless of type of extract, and among the alternatives, 0.1 mg/mL was always better than 10 mg/mL. Ethanol-based extracts with a concentration of 10 mg/mL had very detrimental effects on cell proliferation/migration. At the other two concentrations, ethanol-based extracts had the most beneficial effect, followed by water extracts of fresh leaves, ethanol plus water extracts of dried leaves and, finally, water extracts of dried leaves. CONCLUSIONS: This study suggests that both the water extracts and the more polyphenol-rich ethanol-based extracts of Plantago major leaves have medicinal properties. Further research is, however, needed to determine what compounds are responsible for the wound healing effects.
Authors: Sara H Freiesleben; Jens Soelberg; Nils T Nyberg; Anna K Jäger Journal: Evid Based Complement Alternat Med Date: 2017-02-23 Impact factor: 2.629
Authors: Fernanda de Cássia Israel Cardoso; Priscila Peruzzo Apolinário; Jéssica da Silva Cunha Breder; Thalita Paranhos; Henrique Ceretta Oliveira; Ariane Dini Polidoro; Ana Railka Souza Oliveira Kumakura; Maria Helena Melo Lima Journal: Syst Rev Date: 2019-12-23
Authors: Rabia Mumtaz; Muhammad Zubair; Muhammad Asaf Khan; Saima Muzammil; Muhammad Hussnain Siddique Journal: Evid Based Complement Alternat Med Date: 2022-04-15 Impact factor: 2.650