Literature DB >> 22465330

Application of magnetic hydroxyapatite nanoparticles for solid phase extraction of plasmid DNA.

Zhi Shan1, Xianghai Li, Yaying Gao, Xianxiang Wang, Chenglei Li, Qi Wu.   

Abstract

We developed a facile method for plasmid DNA (pDNA) extraction from crude Escherichia coli lysate using magnetic hydroxyapatite nanoparticles (MHapNPs) in the presence of polyethylene glycol (PEG)/NaCl. DNA condensation induced by PEG/NaCl is a prerequisite for achieving pronounced DNA recovery. The quality and quantity of MHapNP-purified pDNA under optimal binding buffer conditions (0.5 volume of 20% PEG 8000/2M NaCl) were comparable to those obtained using organic solvents or commercial kits. This MHapNP technique is rapid, simple, cost-effective, and environmentally friendly and has the potential to extract DNA from other cell lysates.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 22465330     DOI： 10.1016/j.ab.2012.03.013

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Sonoporation increases therapeutic efficacy of inducible and constitutive BMP2/7 in vivo gene delivery.

Authors:  Georg A Feichtinger; Anna T Hofmann; Paul Slezak; Sebastian Schuetzenberger; Martin Kaipel; Ernst Schwartz; Anne Neef; Nikolitsa Nomikou; Thomas Nau; Martijn van Griensven; Anthony P McHale; Heinz Redl
Journal:  Hum Gene Ther Methods       Date:  2013-11-27       Impact factor: 2.396

2.  Nano-hydroxyapatite/polyaniline composite as an efficient sorbent for sensitive determination of the polycyclic aromatic hydrocarbons in air by a needle trap device.

Authors:  Ali Akbar Alinaghi Langari; Saber Alizadeh; Shiva Soury; Ali Firoozichahak; Davood Nematollahi; Parsa Mohammad Alizadeh; Nasim Sanaei
Journal:  RSC Adv       Date:  2020-11-19       Impact factor: 4.036

Review 3.  Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application.

Authors:  Sudip Mondal; Panchanathan Manivasagan; Subramaniyan Bharathiraja; Madhappan Santha Moorthy; Hye Hyun Kim; Hansu Seo; Kang Dae Lee; Junghwan Oh
Journal:  Int J Nanomedicine       Date:  2017-11-22

4.  Designed Synthesis of Nanostructured Magnetic Hydroxyapatite Based Drug Nanocarrier for Anti-Cancer Drug Delivery toward the Treatment of Human Epidermoid Carcinoma.

Authors:  Bharath Govindan; Beeseti Swarna Latha; Ponpandian Nagamony; Faheem Ahmed; Muheet Alam Saifi; Abdel Halim Harrath; Saleh Alwasel; Lamjed Mansour; Edreese H Alsharaeh
Journal:  Nanomaterials (Basel)       Date:  2017-06-06       Impact factor: 5.076
  4 in total

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