| Literature DB >> 22459351 |
Yingmei Wang1, Yuanxi Zhu, Lizhi Zhang, Wenyan Tian, Shaofang Hua, Jing Zhao, Huiying Zhang, Fengxia Xue.
Abstract
The involvement of insulin in endometrial carcinoma (EC) was investigated using radioimmunoassay, Western blot, immunoprecipitation, MTT, and Annexin V-FITC/PI assays in tissue samples and cultured cells. Serum levels of insulin, p-p52Shc, p-p46Shc, Shc·Grb2 complexes, p-MEK, p-ERK, and cyclin D1 were elevated in patients with EC. Expression of key proteins in the MEK/ERK pathway, including p-p52Shc, Shc·Grb2 complexes, p-MEK, p-ERK, and cyclin D1, was significantly higher in patients with advanced FIGO stage, high grade, and lymph-node metastasis and correlated positively with serum insulin concentration. Insulin promotes Ishikawa 3-H-12 cell proliferation, survival, and invasion, and these effects induced by insulin were significantly blocked by MEK inhibitor PD98059. Insulin thus promotes EC cell proliferation, survival, and invasion via the MEK/ERK pathway.Entities:
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Year: 2012 PMID： 22459351 DOI： 10.1016/j.canlet.2012.03.026
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679