BACKGROUND: The aim of this study was to evaluate alterations in Th1 and Th2 cytokines during experimental abdominal aortic aneurysm (AAA) formation. METHODS: AAAs were induced in apolipoprotein E null mice by infusing angiotensin II (Ang II, 1000 ng/kg/min). Aortic homogenates were assessed at 0, 7, 14, and 28 d (n = 11/time point) for select Th1 and Th2 cytokines by ELISA. Additional mice had co-administration of anti-IgG (n = 20) or anti-IL-5 (n = 20) and were assessed at 28 d for AAA. Aortic homogenates were assessed for MMP-2 and MMP-9 expression. Mouse aortic SMC (MASMC) and peritoneal-derived macrophages were treated with IL-5 (0-40 ng/mL), and cell extracts and media (0-48 h) were assessed for MMP-2 and MMP-9 expression. RESULTS: Ang II infusion was associated with a 3.4-fold (P < 0.01) and 3.6-fold (P < 0.01) increase in IL-5 and IL-10 (respectively), and a 0.6-fold reduction in IL-6, by 7 d. Anti-IL-5, but not anti-IgG, ameliorated Ang II-induced AAA formation. Up-regulation of MMP-2 and MMP-9 was observed in aneurysmal aortas, but not in the aortas obtained from mice treated with anti-IL-5. IL-5 stimulation of MASMC increased MMP-2 and MMP-9 mRNA (2.1-fold and 2.7-fold, respectively, P < 0.01) and protein (1.6-fold and 1.9-fold, respectively, P < 0.01) by 24 h. IL-5 stimulation of macrophages did not alter MMP expression. CONCLUSIONS: Ang II induces increased Th2 cytokines IL-5 and IL-10 early in the course of experimental AAA formation, and inhibition of IL-5 prevents AAA formation suggesting an important role. While IL-5 is capable of up-regulating MMP-2 and MMP-9 expression in MASMC, investigations into alternate roles in AAA formation is warranted.
BACKGROUND: The aim of this study was to evaluate alterations in Th1 and Th2 cytokines during experimental abdominal aortic aneurysm (AAA) formation. METHODS: AAAs were induced in apolipoprotein E null mice by infusing angiotensin II (Ang II, 1000 ng/kg/min). Aortic homogenates were assessed at 0, 7, 14, and 28 d (n = 11/time point) for select Th1 and Th2 cytokines by ELISA. Additional mice had co-administration of anti-IgG (n = 20) or anti-IL-5 (n = 20) and were assessed at 28 d for AAA. Aortic homogenates were assessed for MMP-2 and MMP-9 expression. Mouse aortic SMC (MASMC) and peritoneal-derived macrophages were treated with IL-5 (0-40 ng/mL), and cell extracts and media (0-48 h) were assessed for MMP-2 and MMP-9 expression. RESULTS:Ang II infusion was associated with a 3.4-fold (P < 0.01) and 3.6-fold (P < 0.01) increase in IL-5 and IL-10 (respectively), and a 0.6-fold reduction in IL-6, by 7 d. Anti-IL-5, but not anti-IgG, ameliorated Ang II-induced AAA formation. Up-regulation of MMP-2 and MMP-9 was observed in aneurysmal aortas, but not in the aortas obtained from mice treated with anti-IL-5. IL-5 stimulation of MASMC increased MMP-2 and MMP-9 mRNA (2.1-fold and 2.7-fold, respectively, P < 0.01) and protein (1.6-fold and 1.9-fold, respectively, P < 0.01) by 24 h. IL-5 stimulation of macrophages did not alter MMP expression. CONCLUSIONS:Ang II induces increased Th2 cytokines IL-5 and IL-10 early in the course of experimental AAA formation, and inhibition of IL-5 prevents AAA formation suggesting an important role. While IL-5 is capable of up-regulating MMP-2 and MMP-9 expression in MASMC, investigations into alternate roles in AAA formation is warranted.
Authors: T T Kung; D M Stelts; J A Zurcher; G K Adams; R W Egan; W Kreutner; A S Watnick; H Jones; R W Chapman Journal: Am J Respir Cell Mol Biol Date: 1995-09 Impact factor: 6.914
Authors: Philipp Jud; Nicolas Verheyen; Martin H Stradner; Christian Dejaco; Dieter Szolar; René Thonhofer; Leyla Schweiger; Marianne Brodmann; Franz Hafner Journal: Rheumatol Int Date: 2022-08-23 Impact factor: 3.580
Authors: Cong-Lin Liu; Yi Wang; Mengyang Liao; Holger Wemmelund; Jingyuan Ren; Cleverson Fernandes; Yi Zhou; Galina K Sukhova; Jes S Lindholt; Søren P Johnsen; Jin-Ying Zhang; Xiang Cheng; Xiaozhu Huang; Alan Daugherty; Bruce D Levy; Peter Libby; Guo-Ping Shi Journal: Arterioscler Thromb Vasc Biol Date: 2015-11-05 Impact factor: 8.311
Authors: Miska Sulkava; Emma Raitoharju; Ari Mennander; Mari Levula; Ilkka Seppälä; Leo-Pekka Lyytikäinen; Otso Järvinen; Thomas Illig; Norman Klopp; Nina Mononen; Reijo Laaksonen; Mika Kähönen; Niku Oksala; Terho Lehtimäki Journal: Sci Rep Date: 2017-09-21 Impact factor: 4.379
Authors: Iuliia O Peshkova; Turan Aghayev; Aliia R Fatkhullina; Petr Makhov; Elizaveta K Titerina; Satoru Eguchi; Yin Fei Tan; Andrew V Kossenkov; Marina V Khoreva; Lyudmila V Gankovskaya; Stephen M Sykes; Ekaterina K Koltsova Journal: Nat Commun Date: 2019-11-06 Impact factor: 14.919
Authors: Gijs H M van Puijvelde; Amanda C Foks; Rosemarie E van Bochove; Ilze Bot; Kim L L Habets; Saskia C de Jager; Mariëtte N D Ter Borg; Puck van Osch; Louis Boon; Mariska Vos; Vivian de Waard; Johan Kuiper Journal: PLoS One Date: 2018-01-18 Impact factor: 3.240
Authors: Karlijn B Rombouts; Tara A R van Merrienboer; Johannes C F Ket; Natalija Bogunovic; Jolanda van der Velden; Kak Khee Yeung Journal: Eur J Clin Invest Date: 2021-11-21 Impact factor: 5.722