| Literature DB >> 22454123 |
Zharain Bawa1, Richard A J Darby.
Abstract
A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields. Described here are online and off-line methods to monitor and control methanol addition to bench-top-scale bioreactors.Entities:
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Year: 2012 PMID： 22454123 DOI： 10.1007/978-1-61779-770-5_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745