Literature DB >> 22454110

Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review).

Richard A J Darby1, Stephanie P Cartwright, Marvin V Dilworth, Roslyn M Bill.   

Abstract

Having decided on yeast as a production host, the choice of species is often the first question any researcher new to the field will ask. With over 500 known species of yeast to date, this could pose a significant challenge. However, in reality, only very few species of yeast have been employed as host organisms for the production of recombinant proteins. The two most widely used, Saccharomyces cerevisiae and Pichia pastoris, are compared and contrasted here.

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Year:  2012        PMID: 22454110     DOI: 10.1007/978-1-61779-770-5_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  13 in total

1.  Production of a Hepatitis E Vaccine Candidate Using the Pichia pastoris Expression System.

Authors:  Jyoti Gupta; Amit Kumar; Milan Surjit
Journal:  Methods Mol Biol       Date:  2022

2.  Membrane Protein Production in the Yeast P. pastoris.

Authors:  Hoor Ayub; Michelle Clare; Luke Broadbent; John Simms; Alan D Goddard; Alice J Rothnie; Roslyn M Bill
Journal:  Methods Mol Biol       Date:  2022

3.  Cloning and expression of synthetic genes encoding the broad antimicrobial spectrum bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by recombinant Pichia pastoris.

Authors:  Sara Arbulu; Juan J Jiménez; Loreto Gútiez; Luis M Cintas; Carmen Herranz; Pablo E Hernández
Journal:  Biomed Res Int       Date:  2015-03-02       Impact factor: 3.411

Review 4.  Can too many copies spoil the broth?

Authors:  Rochelle Aw; Karen M Polizzi
Journal:  Microb Cell Fact       Date:  2013-12-20       Impact factor: 5.328

5.  Leveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiae.

Authors:  Yuping Lin; Kulika Chomvong; Ligia Acosta-Sampson; Raíssa Estrela; Jonathan M Galazka; Soo Rin Kim; Yong-Su Jin; Jamie Hd Cate
Journal:  Biotechnol Biofuels       Date:  2014-08-27       Impact factor: 6.040

6.  Active Expression of Human Tissue Plasminogen Activator (t-PA) c-DNA from Pulmonary Metastases in the Methylotrophic Yeast Pichia Pastoris KM71H Strain

Authors:  Amir Hossein Mohseni; Mohammad Soleimani; Keivan Majidzadeh-A; Sedigheh Taghinezhad-S; Hossein Keyvani
Journal:  Asian Pac J Cancer Prev       Date:  2017-08-27

7.  Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor.

Authors:  Anh-Minh Tran; Thanh-Thao Nguyen; Cong-Thuan Nguyen; Xuan-Mai Huynh-Thi; Cao-Tri Nguyen; Minh-Thuong Trinh; Linh-Thuoc Tran; Stephanie P Cartwright; Roslyn M Bill; Hieu Tran-Van
Journal:  BMC Res Notes       Date:  2017-04-04

8.  Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.

Authors:  Juan J Barrero; Alejandro Pagazartaundua; Benjamin S Glick; Francisco Valero; Pau Ferrer
Journal:  N Biotechnol       Date:  2020-10-09       Impact factor: 5.079

9.  An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures.

Authors:  Augusto Q Pedro; Luís M Martins; João M L Dias; Maria J Bonifácio; João A Queiroz; Luís A Passarinha
Journal:  Microb Cell Fact       Date:  2015-08-07       Impact factor: 5.328

10.  Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris.

Authors:  Mikako Tachioka; Naohisa Sugimoto; Akihiko Nakamura; Naoki Sunagawa; Takuya Ishida; Taku Uchiyama; Kiyohiko Igarashi; Masahiro Samejima
Journal:  Biotechnol Biofuels       Date:  2016-09-19       Impact factor: 6.040

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