| Literature DB >> 22454063 |
Jing Wan1.
Abstract
Manipulation of alternative splicing is a unique way of regulating gene expression. Here, a detailed protocol is presented for modification of HER2 pre-mRNA alternative splicing. A 20-mer splice switching oligonucleotide (SSO) targeting exon 15 in HER2 pre-mRNA induced skipping of exon 15, resulted in significant downregulation of full-length HER2 mRNA and protein expression in HER2-overexpressing breast cancer cell line SK-BR-3.Entities:
Mesh：
Substances：
Year: 2012 PMID： 22454063 DOI： 10.1007/978-1-61779-767-5_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745