Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and cellular localization of the Entamoeba histolytica transcarboxylase.

Literature DB >> 22453500

Purification and cellular localization of the Entamoeba histolytica transcarboxylase.

E Barbosa-Cabrera¹, A Salas-Casas, S Rojas-Hernández, A Jarillo-Luna, E Abarca-Rojano, M A Rodríguez, R Campos-Rodríguez.

Abstract

Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.

Entities: Chemical Species

Mesh：

Substances：

Year: 2012 PMID： 22453500 DOI： 10.1007/s00436-012-2898-6

Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289

Keyword Cloud
References

29 in total

1. Crystal structure of the biotin carboxylase domain of human acetyl-CoA carboxylase 2.

Authors: Yong Soon Cho; Jae Il Lee; Dongkyu Shin; Hyun Tae Kim; Young Hoon Cheon; Chang Il Seo; Yong Eun Kim; Young-Lan Hyun; Yoon Sup Lee; Kanako Sugiyama; Sam-Yong Park; Seonggu Ro; Joong Myung Cho; Tae Gyu Lee; Yong-Seok Heo
Journal: Proteins Date: 2008-01-01

2. The genome of the protist parasite Entamoeba histolytica.

Authors: Brendan Loftus; Iain Anderson; Rob Davies; U Cecilia M Alsmark; John Samuelson; Paolo Amedeo; Paola Roncaglia; Matt Berriman; Robert P Hirt; Barbara J Mann; Tomo Nozaki; Bernard Suh; Mihai Pop; Michael Duchene; John Ackers; Egbert Tannich; Matthias Leippe; Margit Hofer; Iris Bruchhaus; Ute Willhoeft; Alok Bhattacharya; Tracey Chillingworth; Carol Churcher; Zahra Hance; Barbara Harris; David Harris; Kay Jagels; Sharon Moule; Karen Mungall; Doug Ormond; Rob Squares; Sally Whitehead; Michael A Quail; Ester Rabbinowitsch; Halina Norbertczak; Claire Price; Zheng Wang; Nancy Guillén; Carol Gilchrist; Suzanne E Stroup; Sudha Bhattacharya; Anuradha Lohia; Peter G Foster; Thomas Sicheritz-Ponten; Christian Weber; Upinder Singh; Chandrama Mukherjee; Najib M El-Sayed; William A Petri; C Graham Clark; T Martin Embley; Bart Barrell; Claire M Fraser; Neil Hall
Journal: Nature Date: 2005-02-24 Impact factor: 49.962

3. Phosphopyruvate carboxylase from entamoeba histolytica.

Authors: R E Reeves
Journal: Biochim Biophys Acta Date: 1970-11-11

4. Molecular cloning and nucleotide sequence of the streptavidin gene.

Authors: C E Argaraña; I D Kuntz; S Birken; R Axel; C R Cantor
Journal: Nucleic Acids Res Date: 1986-02-25 Impact factor: 16.971

Review 5. Acetyl-coenzyme A carboxylases: versatile targets for drug discovery.

Authors: Liang Tong; H James Harwood
Journal: J Cell Biochem Date: 2006-12-15 Impact factor: 4.429

6. An isoleucine/leucine residue in the carboxyltransferase domain of acetyl-CoA carboxylase is critical for interaction with aryloxyphenoxypropionate and cyclohexanedione inhibitors.

Authors: O Zagnitko; J Jelenska; G Tevzadze; R Haselkorn; P Gornicki
Journal: Proc Natl Acad Sci U S A Date: 2001-05-29 Impact factor: 11.205

Purification and cellular localization of the Entamoeba histolytica transcarboxylase.

1. Crystal structure of the biotin carboxylase domain of human acetyl-CoA carboxylase 2.

2. The genome of the protist parasite Entamoeba histolytica.

3. Phosphopyruvate carboxylase from entamoeba histolytica.

4. Molecular cloning and nucleotide sequence of the streptavidin gene.

Review 5. Acetyl-coenzyme A carboxylases: versatile targets for drug discovery.

6. An isoleucine/leucine residue in the carboxyltransferase domain of acetyl-CoA carboxylase is critical for interaction with aryloxyphenoxypropionate and cyclohexanedione inhibitors.

Review 7. Inhibitors of fatty acid synthesis as antimicrobial chemotherapeutics.

Review 8. Regulation of acetyl-CoA carboxylase.

9. A type II pathway for fatty acid biosynthesis presents drug targets in Plasmodium falciparum.

10. Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum.