A Song1, J Cai, K Pan, P Yang. 1. Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
Abstract
OBJECTIVES: To observe whether preserved healthy cementum could promote differentiation of human periodontal ligament cells to cementoblasts. MATERIALS AND METHODS: Symmetrical root slices from each healthy premolar were distributed into either the control group (cementum removed) or test group (cementum preserved). After isolation and characterization, human periodontal ligament cells were inoculated onto root slices for 7 days co-culture. Two slices per group were studied for cell morphology by scanning electronic microscopy. Twenty-three slices were detected for expression of cementum attachment protein and cementum protein 23, two putative cementoblast markers, by real-time polymerase chain reaction. Twenty slices were transplanted into nude mice and analysed using histology and immunohistochemistry for osteopontin and bone sialoprotein expression after 8 weeks. RESULTS: Cells of the test group had smoother fibroblast morphology and higher cementum protein 23 and cementum attachment protein expression than those of the control group (P < 0.01). In the test group, 14 root slices revealed cementum-like matrix formation resting on old cementum; no splits were observed between newly formed matrix and old cementum. In the control group, 17 specimens had fibrous tissue formation along the root surface and varying width of splits could be seen between new fibrous tissue and dentine surface. Only three specimens demonstrated presence of newly formed thin cementum-like matrix. Newly formed cementum-like matrix was positive for osteopontin and bone sialoprotein. CONCLUSIONS: The results demonstrate that healthy root cementum may promote differentiation of human periodontal ligament cells towards cementoblasts.
OBJECTIVES: To observe whether preserved healthy cementum could promote differentiation of human periodontal ligament cells to cementoblasts. MATERIALS AND METHODS: Symmetrical root slices from each healthy premolar were distributed into either the control group (cementum removed) or test group (cementum preserved). After isolation and characterization, human periodontal ligament cells were inoculated onto root slices for 7 days co-culture. Two slices per group were studied for cell morphology by scanning electronic microscopy. Twenty-three slices were detected for expression of cementum attachment protein and cementum protein 23, two putative cementoblast markers, by real-time polymerase chain reaction. Twenty slices were transplanted into nude mice and analysed using histology and immunohistochemistry for osteopontin and bone sialoprotein expression after 8 weeks. RESULTS: Cells of the test group had smoother fibroblast morphology and higher cementum protein 23 and cementum attachment protein expression than those of the control group (P < 0.01). In the test group, 14 root slices revealed cementum-like matrix formation resting on old cementum; no splits were observed between newly formed matrix and old cementum. In the control group, 17 specimens had fibrous tissue formation along the root surface and varying width of splits could be seen between new fibrous tissue and dentine surface. Only three specimens demonstrated presence of newly formed thin cementum-like matrix. Newly formed cementum-like matrix was positive for osteopontin and bone sialoprotein. CONCLUSIONS: The results demonstrate that healthy root cementum may promote differentiation of human periodontal ligament cells towards cementoblasts.
Authors: P F Gonçalves; B C V Gurgel; S P Pimentel; E A Sallum; A W Sallum; M Z Casati; F H Nociti Journal: J Periodontal Res Date: 2006-12 Impact factor: 4.419