Literature DB >> 22447127

Sorting nexins 1 and 2a locate mainly to the TGN.

York-Dieter Stierhof1, Corrado Viotti, David Scheuring, Silke Sturm, David G Robinson.   

Abstract

The subcellular localization of the sorting nexins (SNXs) in higher plants is a matter of controversy. Previous confocal laser scanning microscopy (CLSM studies on root cells from a transgenic Arabidopsis line expressing SNX1-GFP have suggested that this SNX is present on an endosome having characteristics of both the trans-Golgi network (TGN) and the multivesicular body (MVB). In contrast, SNX2a locates exclusively to the TGN when transiently expressed in tobacco mesophyll protoplasts. By performing immunogold electron microscopy on cryofixed Arabidopsis roots, we have tried to clarify the situation. Both SNX1-GFP and endogenous SNX2a locate principally to the TGN. Labeling of MVBs could not be confirmed with any certainty.

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Year:  2012        PMID: 22447127     DOI: 10.1007/s00709-012-0399-1

Source DB:  PubMed          Journal:  Protoplasma        ISSN: 0033-183X            Impact factor:   3.356


  33 in total

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  14 in total

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Review 8.  The plant secretory pathway seen through the lens of the cell wall.

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9.  SORTING NEXIN1 is required for modulating the trafficking and stability of the Arabidopsis IRON-REGULATED TRANSPORTER1.

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