Literature DB >> 22443482

Optimization of pulsed-field gel electrophoresis protocols for Salmonella Paratyphi A subtyping.

Chunxia Chen1, Yingwei Zhao, Hui Han, Bo Pang, Jingyun Zhang, Meiying Yan, Baowei Diao, Zhigang Cui, Haijian Zhou, Weili Liang, Yanfang Feng, Biao Kan.   

Abstract

Salmonella enterica serovar Paratyphi A infection has caused public health problems in some countries in recent years. Pulsed-field gel electrophoresis (PFGE) has been used for the subtyping and epidemiological investigations of some serotypes of Salmonella, mainly in outbreaks caused by non-typhoidal Salmonella. In this study, different restriction endonucleases and electrophoresis parameters were compared for the PFGE subtyping by using Salmonella Paratyphi A strain panels. Two protocols for the enzymes SpeI and XbaI showed higher discriminatory power, which may facilitate epidemiological analysis for more accurate case definition, and clonality study of Salmonella Paratyphi A.

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Year:  2012        PMID: 22443482     DOI: 10.1089/fpd.2011.1023

Source DB:  PubMed          Journal:  Foodborne Pathog Dis        ISSN: 1535-3141            Impact factor:   3.171


  4 in total

1.  Pan-genomic analysis provides insights into the genomic variation and evolution of Salmonella Paratyphi A.

Authors:  Weili Liang; Yongbing Zhao; Chunxia Chen; Xiaoying Cui; Jun Yu; Jingfa Xiao; Biao Kan
Journal:  PLoS One       Date:  2012-09-19       Impact factor: 3.240

2.  A Large-Scale Community-Based Outbreak of Paratyphoid Fever Caused by Hospital-Derived Transmission in Southern China.

Authors:  Meiying Yan; Bo Yang; Zhigang Wang; Shukun Wang; Xiaohe Zhang; Yanhua Zhou; Bo Pang; Baowei Diao; Rusong Yang; Shuyu Wu; John D Klena; Biao Kan
Journal:  PLoS Negl Trop Dis       Date:  2015-07-17

3.  Identification of genes to differentiate closely related Salmonella lineages.

Authors:  Qing-Hua Zou; Ren-Qing Li; Ye-Jun Wang; Shu-Lin Liu
Journal:  PLoS One       Date:  2013-02-18       Impact factor: 3.240

4.  The core proteome and pan proteome of Salmonella Paratyphi A epidemic strains.

Authors:  Li Zhang; Di Xiao; Bo Pang; Qian Zhang; Haijian Zhou; Lijuan Zhang; Jianzhong Zhang; Biao Kan
Journal:  PLoS One       Date:  2014-02-24       Impact factor: 3.240

  4 in total

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