Literature DB >> 22442226

Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian translation elongation factor eEF1A2.

A Yaremchuk1, V F Shalak, O V Novosylna, B S Negrutskii, T Crépin, A V El'skaya, M Tukalo.   

Abstract

Translation elongation factor eEF1A2 was purified to homogeneity from rabbit muscle by two consecutive ion-exchange column-chromatography steps and this mammalian eEF1A2 was successfully crystallized for the first time. Protein crystals obtained using ammonium sulfate as precipitant diffracted to 2.5 Å resolution and belonged to space group P6(1)22 or P6(3)22 (unit-cell parameters a = b = 135.4, c = 304.6 Å). A complete native data set was collected to 2.7 Å resolution.
© 2012 International Union of Crystallography

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Year:  2012        PMID： 22442226      PMCID： PMC3310534          DOI： 10.1107/S1744309112000243

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  25 in total

1.  XDS.

Authors:  Wolfgang Kabsch
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-01-22

2.  Mouse translation elongation factor eEF1A-2 interacts with Prdx-I to protect cells against apoptotic death induced by oxidative stress.

Authors:  Ruying Chang; Eugenia Wang
Journal:  J Cell Biochem       Date:  2007-02-01       Impact factor: 4.429

3.  Characterization of elongation factor-1A (eEF1A-1) and eEF1A-2/S1 protein expression in normal and wasted mice.

Authors:  A Khalyfa; D Bourbeau; E Chen; E Petroulakis; J Pan; S Xu; E Wang
Journal:  J Biol Chem       Date:  2001-04-09       Impact factor: 5.157

4.  The human elongation factor 1 A-2 gene (EEF1A2): complete sequence and characterization of gene structure and promoter activity.

Authors:  C Bischoff; S Kahns; A Lund; H F Jørgensen; M Praestegaard; B F Clark; H Leffers
Journal:  Genomics       Date:  2000-08-15       Impact factor: 5.736

5.  A critical role for eukaryotic elongation factor 1A-1 in lipotoxic cell death.

Authors:  Nica M Borradaile; Kimberly K Buhman; Laura L Listenberger; Carolyn J Magee; Emiko T A Morimoto; Daniel S Ory; Jean E Schaffer
Journal:  Mol Biol Cell       Date:  2005-11-30       Impact factor: 4.138

6.  The utrophin A 5'-UTR drives cap-independent translation exclusively in skeletal muscles of transgenic mice and interacts with eEF1A2.

Authors:  P Miura; A Coriati; G Bélanger; Y De Repentigny; J Lee; R Kothary; M Holcik; B J Jasmin
Journal:  Hum Mol Genet       Date:  2010-01-06       Impact factor: 6.150

7.  Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha.

Authors:  Yvette R Pittman; Kimberly Kandl; Marcus Lewis; Louis Valente; Terri Goss Kinzy
Journal:  J Biol Chem       Date:  2008-12-18       Impact factor: 5.157

8.  Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor.

Authors:  Zhenghe Li; Judit Pogany; Tadas Panavas; Kai Xu; Anthony M Esposito; Terri Goss Kinzy; Peter D Nagy
Journal:  Virology       Date:  2009-01-07       Impact factor: 3.616

9.  Interaction of elongation factor 1-alpha with leucine-rich repeat kinase 2 impairs kinase activity and microtubule bundling in vitro.

Authors:  F Gillardon
Journal:  Neuroscience       Date:  2009-06-25       Impact factor: 3.590

10.  Fine mapping of interactions between eEF1alpha protein and 3'UTR of metallothionein-1 mRNA.

Authors:  Kunbo Fan; Zofia M A Chrzanowska-Lightowlers; John E Hesketh
Journal:  Biochem Biophys Res Commun       Date:  2009-06-06       Impact factor: 3.575
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  4 in total

1.  Mammalian translation elongation factor eEF1A2: X-ray structure and new features of GDP/GTP exchange mechanism in higher eukaryotes.

Authors:  Thibaut Crepin; Vyacheslav F Shalak; Anna D Yaremchuk; Dmytro O Vlasenko; Andrew McCarthy; Boris S Negrutskii; Michail A Tukalo; Anna V El'skaya
Journal:  Nucleic Acids Res       Date:  2014-10-17       Impact factor: 16.971

2.  Oxazole-Benzenesulfonamide Derivatives Inhibit HIV-1 Reverse Transcriptase Interaction with Cellular eEF1A and Reduce Viral Replication.

Authors:  Daniel J Rawle; Dongsheng Li; Zhonglan Wu; Lu Wang; Marcus Choong; Mary Lor; Robert C Reid; David P Fairlie; Jonathan Harris; Gilda Tachedjian; Sally-Ann Poulsen; David Harrich
Journal:  J Virol       Date:  2019-05-29       Impact factor: 5.103

Review 3.  The eEF1A Proteins: At the Crossroads of Oncogenesis, Apoptosis, and Viral Infections.

Authors:  Wasim Abbas; Amit Kumar; Georges Herbein
Journal:  Front Oncol       Date:  2015-04-07       Impact factor: 6.244

4.  Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin.

Authors:  Alejandro Losada; María José Muñoz-Alonso; Carolina García; Pedro A Sánchez-Murcia; Juan Fernando Martínez-Leal; Juan Manuel Domínguez; M Pilar Lillo; Federico Gago; Carlos M Galmarini
Journal:  Sci Rep       Date:  2016-10-07       Impact factor: 4.379
  4 in total

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