| Literature DB >> 22441978 |
Jun-Ming Tang1, Jie Yuan, Qing Li, Jia-Ning Wang, Xia Kong, Fei Zheng, Lei Zhang, Long Chen, Lin-Yun Guo, Yong-Hang Huang, Jian-Ye Yang, Shi-You Chen.
Abstract
Acetylcholine (ACh) plays an important role in neural and non-neural function, but its role in mesenchymal stem cell (MSC) migration remains to be determined. In the present study, we have found that ACh induces MSC migration via muscarinic acetylcholine receptors (mAChRs). Among several mAChRs, MSCs express mAChR subtype 1 (m1AChR). ACh induces MSC migration via interaction with mAChR1. MEK1/2 inhibitor PD98059 blocks ERK1/2 phosphorylation while partially inhibiting the ACh-induced MSC migration. InsP3Rs inhibitor 2-APB that inhibits MAPK/ERK phosphorylation completely blocks ACh-mediated MSC migration. Interestingly, intracellular Ca(2+) ATPase-specific inhibitor thapsigargin also completely blocks ACh-induced MSC migration through the depletion of intracellular Ca(2+) storage. PKCα or PKCβ inhibitor or their siRNAs only partially inhibit ACh-induced MSC migration, but PKC-ζ siRNA completely inhibits ACh-induced MSC migration via blocking ERK1/2 phosphorylation. These results indicate that ACh induces MSC migration via Ca(2+), PKC, and ERK1/2 signal pathways.Entities:
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Year: 2012 PMID： 22441978 PMCID： PMC4263956 DOI： 10.1002/jcb.24148
Source DB: PubMed Journal: J Cell Biochem ISSN： 0730-2312 Impact factor: 4.429