BACKGROUND: Debridement is one of the crucial steps for successful wound care. In addition to removing necrotic tissue, debridement has been shown to reduce wound-associated bacteria that delay healing. Using an in vivo porcine model, we compared the effects of various methods of debridement, including hydrosurgery and plasma-mediated bipolar radiofrequency ablation (PBRA), on bacterial removal and wound healing. METHODS: One hundred thirty-five deep dermal wounds were inoculated with methicillin resistant Staphylococcus aureus (MRSA) and covered with a polyurethane dressing for 48 h to allow for biofilm formation. Wounds were then treated with either PBRA (at two settings), hydrosurgery, sharp debridement, or no debridement. Biopsies were collected for microbiology and histologic assessment on d 0, 2, 9, and 21 post-treatment. RESULTS: All treatment groups showed a statistically significant reduction in MRSA counts relative to no debridement at all times points (P < 0.05). PBRA at a maximum setting had the lowest MRSA counts at all recovery times and, compared with all other treatment groups, a statistically significant difference was observed on d 21 (P < 0.05). No detrimental effects on the healing process were noted with any of the debridement methods. CONCLUSION: While sharp debridement has been established as the traditional gold standard for rapid removal of necrotic, infected tissue, our results suggest that novel debridement modalities show clinical promise for the treatment of chronic ulcers and burn wounds, especially when bacteria are present.
BACKGROUND: Debridement is one of the crucial steps for successful wound care. In addition to removing necrotic tissue, debridement has been shown to reduce wound-associated bacteria that delay healing. Using an in vivo porcine model, we compared the effects of various methods of debridement, including hydrosurgery and plasma-mediated bipolar radiofrequency ablation (PBRA), on bacterial removal and wound healing. METHODS: One hundred thirty-five deep dermal wounds were inoculated with methicillin resistant Staphylococcus aureus (MRSA) and covered with a polyurethane dressing for 48 h to allow for biofilm formation. Wounds were then treated with either PBRA (at two settings), hydrosurgery, sharp debridement, or no debridement. Biopsies were collected for microbiology and histologic assessment on d 0, 2, 9, and 21 post-treatment. RESULTS: All treatment groups showed a statistically significant reduction in MRSA counts relative to no debridement at all times points (P < 0.05). PBRA at a maximum setting had the lowest MRSA counts at all recovery times and, compared with all other treatment groups, a statistically significant difference was observed on d 21 (P < 0.05). No detrimental effects on the healing process were noted with any of the debridement methods. CONCLUSION: While sharp debridement has been established as the traditional gold standard for rapid removal of necrotic, infected tissue, our results suggest that novel debridement modalities show clinical promise for the treatment of chronic ulcers and burn wounds, especially when bacteria are present.
Authors: Stephen C Davis; Andrew Harding; Joel Gil; Fernando Parajon; Jose Valdes; Michael Solis; Alex Higa Journal: Int Wound J Date: 2017-03-07 Impact factor: 3.315
Authors: Jing Liu; Jason H Ko; Erwin Secretov; Eric Huang; Christiana Chukwu; Julie West; Katherine Piserchia; Robert D Galiano Journal: Int Wound J Date: 2013-10-31 Impact factor: 3.315
Authors: Maria Magana; Christina Sereti; Anastasios Ioannidis; Courtney A Mitchell; Anthony R Ball; Emmanouil Magiorkinis; Stylianos Chatzipanagiotou; Michael R Hamblin; Maria Hadjifrangiskou; George P Tegos Journal: Clin Microbiol Rev Date: 2018-04-04 Impact factor: 26.132
Authors: Kenneth S Brandenburg; Diego F Calderon; Patricia R Kierski; Amanda L Brown; Nihar M Shah; Nicholas L Abbott; Michael J Schurr; Christopher J Murphy; Jonathan F McAnulty; Charles J Czuprynski Journal: Wound Repair Regen Date: 2015-11-04 Impact factor: 3.617
Authors: Irena Pastar; Aron G Nusbaum; Joel Gil; Shailee B Patel; Juan Chen; Jose Valdes; Olivera Stojadinovic; Lisa R Plano; Marjana Tomic-Canic; Stephen C Davis Journal: PLoS One Date: 2013-02-22 Impact factor: 3.240