Li-hua Jiang1,2, Ling-ling Wang3,4, Ming-yan Wang5, Hao-xin Wu5, Yi-jie Zou6, Xiao-lin Yuan5, Mei-juan Chen5. 1. Cultivating Candidate of the State Key Laboratory of Integrated Acupuncture and Medication, Nanjing University of Chinese Medicine, Nanjing, 210029, China. birddingdang@yahoo.com.cn. 2. The Second Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210029, China. birddingdang@yahoo.com.cn. 3. Cultivating Candidate of the State Key Laboratory of Integrated Acupuncture and Medication, Nanjing University of Chinese Medicine, Nanjing, 210029, China. 4. The Second Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210029, China. 5. Basic Medical College of Nanjing University of Chinese Medicine, Nanjing, 210029, China. 6. Jiangsu Hospital of Chinese Medicine, Nanjing, 210029, China.
Abstract
OBJECTIVE: To investigate whether pinching spine (PS, i.e. , a traditional Chinese manipulative therapy) is beneficial to ameliorating the depressive state (including behavioral deficit, retardative weight gain and decreased sucrose consumption) in a rat model of depression induced by chronic unpredictable stress (CUS) and to explore the candidate mechanism of action. METHODS: PS was performed on rats' spine once daily for 1 week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were applied on different dates: before stress (d0), at the end of stress (d21) and after PS treatment (d28), respectively. Then the rats' hippocampuses were performed genome-wide microarray analysis, and the expression levels of several genes were evaluated by real-time polymerase chain reaction (PCR). RESULTS: Exposure to CUS resulted in decreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment. The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., were down-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restriction of activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatment significantly inverted these changes. Furthermore, increase or decrease in gene expression evaluated by realtime PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis. CONCLUSION: PS showed a potential antidepressant-like effect, of which the action mechanism might be due to gene expression regulation in hippocampus.
OBJECTIVE: To investigate whether pinching spine (PS, i.e. , a traditional Chinese manipulative therapy) is beneficial to ameliorating the depressive state (including behavioral deficit, retardative weight gain and decreased sucrose consumption) in a rat model of depression induced by chronic unpredictable stress (CUS) and to explore the candidate mechanism of action. METHODS:PS was performed on rats' spine once daily for 1 week after exposure to CUS. The open-field test, body weight measuring, and sucrose intake test were applied on different dates: before stress (d0), at the end of stress (d21) and after PS treatment (d28), respectively. Then the rats' hippocampuses were performed genome-wide microarray analysis, and the expression levels of several genes were evaluated by real-time polymerase chain reaction (PCR). RESULTS: Exposure to CUS resulted in decreases of behavioral activity and sucrose consumption, which were reversed significantly after PS treatment. The expression of several genes relevant to energy metabolism, anti-oxidation, and olfactory receptor, etc., were down-regulated, while the expression of those relevant to hemostasis, immunity-inflammation, and restriction of activities and ingestion, etc., were up-regulated in hippocampuses of rats exposed to CUS. PS treatment significantly inverted these changes. Furthermore, increase or decrease in gene expression evaluated by realtime PCR was concordant with up-regulated or down-regulated expression evaluated by microarray analysis. CONCLUSION:PS showed a potential antidepressant-like effect, of which the action mechanism might be due to gene expression regulation in hippocampus.
Authors: Olga Pollatos; Jessica Albrecht; Rainer Kopietz; Jennifer Linn; Veronika Schoepf; Anna Maria Kleemann; Tatjana Schreder; Rainer Schandry; Martin Wiesmann Journal: J Affect Disord Date: 2007-02-08 Impact factor: 4.839
Authors: Yoshiyuki Satake; Bruno L Diaz; Barbara Balestrieri; Bing K Lam; Yoshihide Kanaoka; Michael J Grusby; Jonathan P Arm Journal: J Biol Chem Date: 2004-02-04 Impact factor: 5.157