OBJECTIVES: To clarify the association of kallikrein-related peptidase 3 (KLK3) rs2735839 G/A polymorphism with serum prostate-specific antigen (PSA) levels in Japanese men. METHODS: Subjects were participants of the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study who visited the Seirei Preventive Health Care Center in Shizuoka, Japan. Among the 5,040 individuals aged 35-69 years who were enrolled in 2006-2007, serum PSA data were available for 2,323 male subjects without a past history of prostate cancer. The diagnostic criteria for PSA positivity was PSA ≥ 4.0 ng/ml. Genotyping of the KLK3 polymorphism was conducted by the polymerase chain reaction with the confronting two-pair primers (PCR-CTPP) method. RESULTS: The mean ± SD of PSA levels (mg/dl) were 1.54 ± 1.73 for those with KLK3 rs2735839 G/G genotype, 1.34 ± 1.33 for G/A, and 1.20 ± 1.23 for A/A, which was significantly different (p < 0.0001). The age-adjusted odds ratios of PSA test positivity were 0.62 (95% confidence interval 0.41-0.94) for those with G/A + A/A relative to those with G/G. CONCLUSIONS: The present study revealed that the KLK3 rs2735839 G allele was significantly associated with higher serum PSA levels also in Japanese.
OBJECTIVES: To clarify the association of kallikrein-related peptidase 3 (KLK3) rs2735839 G/A polymorphism with serum prostate-specific antigen (PSA) levels in Japanese men. METHODS: Subjects were participants of the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study who visited the Seirei Preventive Health Care Center in Shizuoka, Japan. Among the 5,040 individuals aged 35-69 years who were enrolled in 2006-2007, serum PSA data were available for 2,323 male subjects without a past history of prostate cancer. The diagnostic criteria for PSA positivity was PSA ≥ 4.0 ng/ml. Genotyping of the KLK3 polymorphism was conducted by the polymerase chain reaction with the confronting two-pair primers (PCR-CTPP) method. RESULTS: The mean ± SD of PSA levels (mg/dl) were 1.54 ± 1.73 for those with KLK3rs2735839 G/G genotype, 1.34 ± 1.33 for G/A, and 1.20 ± 1.23 for A/A, which was significantly different (p < 0.0001). The age-adjusted odds ratios of PSA test positivity were 0.62 (95% confidence interval 0.41-0.94) for those with G/A + A/A relative to those with G/G. CONCLUSIONS: The present study revealed that the KLK3rs2735839 G allele was significantly associated with higher serum PSA levels also in Japanese.
Authors: Brian T Helfand; Kimberly A Roehl; Phillip R Cooper; Barry B McGuire; Liesel M Fitzgerald; Geraldine Cancel-Tassin; Jean-Nicolas Cornu; Scott Bauer; Erin L Van Blarigan; Xin Chen; David Duggan; Elaine A Ostrander; Mary Gwo-Shu; Zuo-Feng Zhang; Shen-Chih Chang; Somee Jeong; Elizabeth T H Fontham; Gary Smith; James L Mohler; Sonja I Berndt; Shannon K McDonnell; Rick Kittles; Benjamin A Rybicki; Matthew Freedman; Philip W Kantoff; Mark Pomerantz; Joan P Breyer; Jeffrey R Smith; Timothy R Rebbeck; Dan Mercola; William B Isaacs; Fredrick Wiklund; Olivier Cussenot; Stephen N Thibodeau; Daniel J Schaid; Lisa Cannon-Albright; Kathleen A Cooney; Stephen J Chanock; Janet L Stanford; June M Chan; John Witte; Jianfeng Xu; Jeannette T Bensen; Jack A Taylor; William J Catalona Journal: Hum Genet Date: 2015-02-26 Impact factor: 4.132